检验医学与临床2017，Vol.14Issue(23)：3483-3486,4.DOI:10.3969/j.issn.1672-9455.2017.23.021
秦皮乙素对产KPC碳青霉烯耐药肺炎克雷伯菌的体外抗菌效果
Effect of Esculetin inhibiting for KPC-producing Klebsiella pneumoniae in vitro
王蕾 1刘志远 2潘健 2张婷菊 2齐杰 2张国军1
作者信息
- 1. 首都医科大学附属北京天坛医院检验科,北京100050
- 2. 中国中医科学院广安门医院检验科,北京100053
- 折叠
摘要
Abstract
Objective To examine the effect of bacteriostasis of Esculetin against Klebsiella pneumoniae carbapenemase(KPC)-producing Klebsiella pneumoniae.Methods Modified Hodge test,meropenem combined with 3-aminophenylboronic acid(APBA) and EDTA were conducted to detect carbapenemases.KPC gene and sequencing were determined by PCR.The molecular typing of strains was analyzed by MLST-PCR.Minimal inhibitory concentrations(MICs) of Esculetin against the strains for research were de termined by broth dilution in the 96 well microplates.Synergy effects between Esculetin and antibiotic(imipenem,gentamycin,levoloxacin) were designed with chessboard test.Results Modified Hodge Test and APBA inhibiting test were positive in all strains.All samples were KPC-2-producing Klebsiella pneumoniae,and predominantly to a single sequence type,ST11.The tests showed the MICs of Esculetin were 0.5-1.0 mg/mL.The result was FIC higher than 2 in the combination of Esculetin with imipenem in all strains.Different results were observed in the combination between Esculetin and gentamicin according to the MIC of gentamicin.FIC was equal to 2 when the MIC higher than 1 024 μg/mL.FIC was in the range of 0.3-1.0 when MIC was lower than 512 μg/mL.The combination between Esculein and levoloxacin obtained that FIC was equal to 2 in all specimens.Conclusion Esculein has obvious antibacterial effect on KPC-producing Klebsiella pneunoniae.The antagonistic effect is observed in the combination between Esculein and imipenem.The combined efficacy of Esculein and gentamicin are different according to MICs of gentamicin.And there is indifferent action between Esculein and levoloxacin.关键词
秦皮乙素/碳青霉烯耐药肺炎克雷伯菌/抗菌作用Key words
Esculetin/carbapenem resistant Klebsiella pneumoniae/bacteriostasis引用本文复制引用
王蕾,刘志远,潘健,张婷菊,齐杰,张国军..秦皮乙素对产KPC碳青霉烯耐药肺炎克雷伯菌的体外抗菌效果[J].检验医学与临床,2017,14(23):3483-3486,4.
