检验医学与临床2018，Vol.15Issue(5)：657-659,663,4.DOI:10.3969/j.issn.1672-9455.2018.05.026
湿润生肌膏对肛瘘术后创面肉芽组织中碱性成纤维细胞生长因子mRNA表达的影响
Influence of Shengji ointment on expression of basic fibroblast growth factor mRNA in granulation tissue after operation of anal fistula
王世霞 1陈永乐 1王亚儒 1吕高波1
作者信息
- 1. 陕西省宝鸡市中心医院肛肠外科 721008
- 折叠
摘要
Abstract
Objective To explore the efficacy of Shengji ointment on basic fibroblast growth factor mRNA expression of granulation tissue of patients with anal fistula after surgery.Methods From January 2016 to October 2016,95 patients with simple low anal fistula in our hospital accepted surgical treatment were selected as the research subjects.And they were randomly divided into observation group,control group and vaseline group in different period.After the total RNA was extracted from the tissue of the three groups of patients, RNA was purified by Poly A Tract(R)from mRNA isolation system.Meanwhile,using RT-PCR method,the expression of basic fibroblast grow th factor mRNA was observed,and the effect of different groups of patients were compared.Results Wound healing rates of seven days,14 days and 21 days after surgery of observation group were(51.72 ± 4.33)%,(63.85 ± 6.26)% and(95.51 ± 2.79)%,respectively.Compared with the con-trol group and the vaseline group,differences were statistically significant(P<0.05).A total of 29 cases were cured(90.63%)and 3 cases were effective(9.37%)in the observation group,and the treatment effect was better than the control group and the vaseline group patients(P<0.05).Seven days after surgery,basic fibro-blast growth factor mRNA expressions of three groups patients were the strongest,and the basic fibroblast grow th factor mRNA expression of cytoplasm of vascular endothelial cells,fibroblasts and mononuclear cells of the observation group were higher than the other two groups.Conclusion The application of moist Shengji ointment treatment has effect on the expression of basic fibroblast growth factor mRNA in wound granulation tissue in anal fistula after operation.In the process of wound healing,the positive expression of basic fibroblast grow th factor mRNA could promote the migration and proliferation of repair cells and accelerate wound repair ability.关键词
湿润生肌膏/肛瘘/成纤维细胞/生长因子/mRNAKey words
moist ointment/anal fistula/fibroblast/grow th factor/mRNA分类
中医学引用本文复制引用
王世霞,陈永乐,王亚儒,吕高波..湿润生肌膏对肛瘘术后创面肉芽组织中碱性成纤维细胞生长因子mRNA表达的影响[J].检验医学与临床,2018,15(5):657-659,663,4.
