临床肝胆病杂志2018，Vol.34Issue(2)：309-313,5.DOI:10.3969/j.issn.1001-5256.2018.02.018
长链非编码RNA XLOC-007123在乙型肝炎相关肝细胞癌中的表达及临床意义
Expression of the long non-coding RNA XLOC-007123 in hepatitis B-related hepatocellular carcinoma and its clinical significance
雷光林 1王权 1程思杰 1赖成材 2王科宇 2严锦 1杨鹏辉 1张绍庚1
作者信息
- 1. 解放军第三○二医院肝胆外科中心,北京100039
- 2. 军事医学科学院微生物流行病研究所,北京100071
- 折叠
摘要
Abstract
Objective To investigate the expression of the long non-coding RNA (lncRNA) XLOC-007123 in cancer and adjacent tissue,as well as hepatoma cell lines,in patients with hepatitis B-related hepatocellular carcinoma (HCC),and the association between its expression level and clinicopathological features.Methods A total of 52 patients with hepatitis B-related HCC who underwent resection of HCC in Center of Hepatobiliary Surgery,302 Hospital of PLA,from December 2013 to December 2015 were enrolled,and HCC tissue samples were collected.Adjacent tissue samples collected from the same patient were used as controls.Quantitative real-time PCR was used to measure the expression of the lncRNA XLOC-007123 in HCC tissue,adjacent tissue,and hepatoma cell lines in the patients with hepatitis B-related HCC.Normal liver cell line LO2 and hepatoma cell lines HuH7,HepG2,and SMMC-7721 were obtained from Research Center for Clinical and Translational Medicine,302 Hospital of PLA.The nonparametric Wilcoxon rank sum test was used to compare the expression of the IncRNA XLOC-007123 between HCC tissue and adjacent tissue,and the independent samples t-test was used to compare the expression of the lncRNA XLOC-007123 between HCC cell lines and the normal liver cell line.The chi-square test was used for comparison of clinical indices.Results In the patients with hepatitis B-related HCC,the expression of the lncRNA XLOC-007123 was significantly lower in HCC tissue than in adjacent tissue (P ＜ 0.01);also,the expression of the lncRNA XLOC-007123 was significantly lower in HuH7,HepG2,and SMMC-7721 cells than in LO2 cells (all P ＜0.05).The expression of the lncRNA XLOC-007123 was associated with the serum level of alpha-fetoprotein (AFP) and tumor stage (x2 =7.738 and 7.589,both P ＜ 0.05).Conclusion There is a reduction in the expression of the lncRNA XLOC-007123 in hepatitis B-related HCC tissue,which is associated with the serum level of AFP and pathological staging,suggesting that the lncRNA XLOC-007123 may exert a regulatory effect in the development and progression of hepatitis B-related HCC and may become a potential marker for the diagnosis of hepatitis B-related HCC.关键词
癌,肝细胞/肝炎,乙型/RNA/基因表达Key words
carcinoma, hepatocellular/hepatitis B/RNA/gene expression分类
医药卫生引用本文复制引用
雷光林,王权,程思杰,赖成材,王科宇,严锦,杨鹏辉,张绍庚..长链非编码RNA XLOC-007123在乙型肝炎相关肝细胞癌中的表达及临床意义[J].临床肝胆病杂志,2018,34(2):309-313,5.
