农业环境科学学报2018，Vol.37Issue(2)：268-275,8.DOI:10.11654/jaes.2017-0960
铅对塿土细菌群落组成的影响研究
Influence of lead on bacterial community composition in Lou soil
摘要
Abstract
To research the soil bacteria microbial community composition influenced by lead, while examining its relationship with the physicochemical properties of Pb-contaminated Lou soils,the Illumina platform Miseq high-throughput sequencing technique was used to analyze the agricultural soil treated with various concentrations of Pb2+.Three treatments, namely high concentration treatment(WHIGH), low concentration treatment(WLOW), and control(WCK), were used. The results showed that Proteobacteria(with an average relative abundance of 25%)and Actinobacteria(with an average relative abundance of 18.6%)were the most abundant phyla among the three treat-ments. The other dominant phyla were Acidobacteria, Bacteroidetes, etc. Actinobacteria, Betaproteobacteria, and Cytophagi were the most abundant classes in WHIGH among the three treatments.At the genus level,the relative abundance of 9 genera were significantly different among the three treatments(P<0.05), namely Pseudomonas, Skemanella, Balneimona, Agromyces, Enhydrobacter, Devosia, Variovorax, Flavihumibacter and Lacibacter.Moreover,the relative abundance of 7 genera were below 0.3%,except for Pseudomonas and Skemanella in both treatments.The variance analysis showed that the richness index of the community(Chao and ACE)had significant differences between the WHIGH and WLOW treatments(P<0.05).The bacteria community structure of OTU was fairly similar in the WCK and WLOW treat-ments,while it was different in the WHIGH treatment.The redundancy analysis and correlation analysis showed that the content of available Pb and total Pb were positively correlated with WHIGH′s bacterial community structure of OTU,while being negatively correlated with those of WLOW and WCK. Firmicutes, Gemmatimonadetes, Verrucomicrobia,Chloroflexi, and Nitrospirae correlated closely with the physico-chemical properties of soil(P<0.05).The results of the present study indicated that bacterial community of Lou soil has been greatly influ-enced by long-term Pb contamination,which changed the bacterial community composition of the soil.关键词
16SrDNA基因测序/细菌/塿土/铅/细菌群落Key words
16S rDNA gene sequence/bacteria/Lou soil/lead/bacterial community分类
资源环境引用本文复制引用
安凤秋,吕家珑,刁展,李海红,赵琪琪..铅对塿土细菌群落组成的影响研究[J].农业环境科学学报,2018,37(2):268-275,8.基金项目
农业部公益性行业(农业)科研专项(200903015) （农业）
陕西省农业厅环保站项目 （）
陕西省"三秦学者"项目Special-research for Ministry of Agriculture China(200903015) （200903015）
Project for Environmental Protection Station of Ministry of Agriculture in Shaanxi Province （）
Project for“Sanqin Scholar”of Shannxi Province （）
