山东农业科学2017，Vol.49Issue(11)：64-68,5.DOI:10.14083/j.issn.1001-4942.2017.11.012
铁载体产生菌Paenibacillus illinoisensis YZ29在花生根际定殖能力研究
Colonization Ability of Siderophore-Producing Bacterium Paenibacillus illinoisensis YZ29 in Peanut Rhizosphere
摘要
Abstract
Marking target microorganisms with green fluorescent protein (GFP) is an important means of studying the interaction between microorganisms and hosts.In this study,the shuttle vector pGFP4412 was introduced into the siderophore-producing bacterium Paenibacillus illinoisensis YZ29 by electroporation,and the YZ29-gfp strain successfully expressing GFP was obtained.The colonization of YZ29 on the peanut root surface and in the soil was studied by double-resistant plate screening combining with fluorescence microscope observation.The results showed that the green fluorescence could be observed under the blue light with excitation wavelength of 488 nm.The YZ29-gfp could colonize in the peanut rhizosphere and non-rhizo-sphere soils under pot culture.Under the condition of laboratory culture,it could colonize on the peanut root surface and could not colonize in the root.This showed that YZ29 could effectively colonize in the peanut rhizosphere,which layed a foundation for growth-promoting and biocontrol effects of peanut.关键词
铁载体产生菌/伊利诺伊类芽孢杆菌/花生/定殖Key words
Siderophore-producing bacterium/Paenibacillus illinoisensis/Peanut/Colonization分类
农业科技引用本文复制引用
李玉菲,王梅,杜秉海,汪城墙,姚良同,丁延芹..铁载体产生菌Paenibacillus illinoisensis YZ29在花生根际定殖能力研究[J].山东农业科学,2017,49(11):64-68,5.基金项目
山东省科技重大专项(2015ZDXX0502B02) （2015ZDXX0502B02）
中国博士后基金项目(2015M582121) （2015M582121）
校企联合基金项目(史丹利集团) （史丹利集团）
