山东医药2018，Vol.58Issue(3)：5-9,5.DOI:10.3969/j.issn.1002-266X.2018.03.002
S100 A6基因作用对宫颈癌细胞增殖迁移能力的影响及其机制
Effects of S100A6 transfection and inhibition on proliferation and migration of human cervical cancer cells
摘要
Abstract
Objective To observe the effects of S100A6 transfection and inhibition on the proliferation and migration of human cervical cancer cells,and to explore the mechanism.Methods The cervical cancer Hela cells were divided into the observation group 1,the control group 1,and the blank group 1.The observation group 1 was transfected by the pcD-NA3.0-S100A6 eukaryotic expressive vector,the control group 1 was transfected by the empty vector,and the blank group 1 was not transfected.The cervical cancer CaSki cells were divided into the observation group 2,the control group 2,and the blank group 2.The observation group 2 was transfected by the S100A6 siRNA,the control group 2 was transfected by the siRNA-negative controls and the blank group 2 was not transfected.At 24 h after transfection,the cells were collected.①The real-time PCR assay was used to detect the relative expression levels of S 100A6 mRNA in each group.②The cell migration assay was employed to detect the cell migration(the number of migration cells).③Western blotting was utilized to determine the levels of the epithelial-mesenchymal transition(EMT)-related proteins(E-cadherin and N-cadherin),and the levels of the PI3K-Akt signing pathway-related proteins(p-Akt,t-Akt,Snail,and Twist).④At 24,48,and 72 h af-ter 24-hour transfection,CCK-8 was applied to analyze the cell proliferation ability(OD450).Results ① At 24 h after the transfection,the relative expression levels of S100A6 mRNA in Hela cells of the observation group 1,the control group 1,and the blank group 1 were 3.87 ±0.86,1.04 ±0.08, and 0.97 ±0.11, respectively.Compared with the control group 1 and the blank group 1,the relative expression level of S100A6 mRNA in the observation group 1 was higher(P<0.05).The relative expression levels of S100A6 mRNA in the CaSki cells of the observation group 2,the control group 2, and the blank group 2 were 0.43 ±0.07,1.12 ±0.09,and 0.98 ±0.12,respectively.Compared with the control group 2 and the blank group 2,the relative expression level of S100A6 mRNA in the observation group 2 was lower(P<0.05).②At 24 h after the transfection,the migration cells in the observation group 1, the control group 1, and the blank group 1 were 48.26 ±4.89,21.31 ±4.27, and 20.12 ±6.23, respectively.Compared with the control group 1 and the blank group 1,the number of migration cells in the observation group 1 was higher(P<0.05).The migration cells in the obser-vation group 1,the control group 1,and the blank group 1 were 81.36 ±8.33,147.49 ±6.98,and 142.23 ±9.16,re-spectively.Compared with the control group 2 and the blank group 2,the number of migration cells in the observation group 2 was lower(P<0.05).③At 24 h,compared with the control group 1 and the blank group 1,the protein expression of E-cadherin in the observation group 1 was lower(P<0.05), while the protein expression of N-cadherin, p-AKT,Snail, and Twist was higher(all P<0.05).Compared with the control group 2 and the blank group 2,the protein expression of E-cadherin in the observation group 2 was higher(P<0.05),while the protein expression of N-cadherin,p-AKT,Snail, and Twist was lower(all P<0.05).④At 24 and 48 h after 24-hour transfection,the OD450in each group was not differ-ent(all P>0.05).At 72 h,the OD450of Hela cells in the observation group 1 was higher than that in the control group 1 and the blank group 1(P<0.05);the OD450of the CaSki cells in the observation group 2 was lower than that in the con-trol group 2 and the blank group 2(P<0.05).Conclusion S100A6 could promote the proliferation and migration of hu-man cervical cancer cells,which may be achieved by activating the PI 3K-Akt pathway and enhancing the EMT of cervical cancer cells.关键词
S100A6蛋白/宫颈癌/上皮间质转化/PI3K-Akt信号通路Key words
S100A6 protein/cervical carcinoma/epithelial-mesenchymal transition/PI3K-Akt signaling pathway分类
医药卫生引用本文复制引用
姚月荣,刘富群..S100 A6基因作用对宫颈癌细胞增殖迁移能力的影响及其机制[J].山东医药,2018,58(3):5-9,5.基金项目
辽宁省自然科学基金项目(2014021073). （2014021073）
