山东医药2018，Vol.58Issue(3)：15-18,4.DOI:10.3969/j.issn.1002-266X.2018.03.004
缺氧微环境中HIF-1α基因沉默的人绒毛膜癌细胞系JE G-3侵袭和迁移能力观察
Effects of silencing HIF-1αon invasion and migration of human choriocarcinoma JEG-3 cells under hypoxia
摘要
Abstract
Objective To investigate the effects of hypoxia inducible factor 1α(HIF-1α)on the invasion and migra-tion of human choriocarcinoma JEG-3 cells under hypoxia.Methods JEG-3 cells in the logarithmic phase were divided into groups A,B,C,and D;and cells in the groups A,B,and C were cultured under hypoxia in vitro,but the group D was not treated.When JEG-3 cells were fused to 60%to 70%,cells in the groups A and C were transfected with HIF-1α siRNA and NC siRNA,respectively,and we did not do the treatment on the cells in the groups B and D.Real-time PCR and Western blotting were used to detect the mRNA and protein expression levels of HIF -1αand CXCR4 in each group, respectively.The invasion and migration abilities of JEG-3 cells were separately analyzed by Transwell invasion assay and Scratch test.Results The invasion and migration abilities of JEG-3 cells in the group A were significantly lower than those in the other 3 groups(all P<0.05),the invasion and migration abilities of JEG-3 cells in the groups B and C were higher than those in the group D(all P<0.05).The mRNA and protein expression levels of HIF-1αand CXCR4 in the group A were significantly lower than those in the other 3 groups(all P<0.05),while the mRNA and protein expression levels of HIF-1αand CXCR4 in the groups B and C were significantly higher than those in the group D(all P<0.05). Conclusion Silencing HIF-1αcan inhibit the invasion and migration abilities of of JEG-3 cells under hypoxia, and its molecular mechanism may be related to the down-regulation of CXCR4.关键词
缺氧诱导因子1α/趋化因子受体4/滋养细胞/细胞迁移/细胞侵袭/子痫/缺氧微环境Key words
hypoxia-inducible factor 1α/chemokine receptor 4/trophoblast cells/cell migration/cell invasion/ec-lampsia/hypoxia microenvironment分类
医药卫生引用本文复制引用
张展,李鹏云,闫欢,王艳,余洁..缺氧微环境中HIF-1α基因沉默的人绒毛膜癌细胞系JE G-3侵袭和迁移能力观察[J].山东医药,2018,58(3):15-18,4.基金项目
郑州市科技创新项目(131PCXTD624). （131PCXTD624）
