山东医药2018，Vol.58Issue(3)：23-26,4.DOI:10.3969/j.issn.1002-266X.2018.03.006
长期氯化锰腹腔注射母鼠的子代雄鼠生精细胞凋亡变化及其机制探讨
Long-term injection of manganese chloride in mother rats induces apoptosis of spermatogenic cells of offspring rats
摘要
Abstract
Objective To investigate the effects of long-term administration of manganese chloride in mother rats on the apoptosis of spermatogenic cells in testes of offspring male rats.Methods Thirty-two healthy female SD rats were ran-domly divided into the observation groups 1,2,and 3,and the control group,with 8 rats in each group.Then 2,4,and 8 mg/kg MnCl2· 4H2O,and equal volume of normal saline were injected intraperitoneally for 8 weeks(once a day,a con-tinuous injection of 5 days,and 2 days of rest).After mating with normal male SD rats and being identified conception,fe-male rats were continued to be exposed to manganese during pregnancy and lactation.Eight offspring male rats of each group,12 weeks old,were randomly executed and their testes were obtained to observe the structure of seminiferous tubules by H.E staining.The apoptotic index(AI)of spermatogenic cells was measured by TUNEL method.The levels of reactive oxygen species(ROS)in testicles were detected by Chemiluminescence method.The expression of GRP78, CHOP, Caspase-12 and Caspase-3 proteins was detected by Western blotting in the testicles of offspring male rats.Results The AI of the observation groups 1,2,3,and control group were 9.05%±1.02%,15.87%±1.31%,19.59%±1.12%,and 5.72%±0.53%,respectively;significant difference was found in AI between every two groups(all P<0.05).The ROS levels(expressed as fluorescence intensity)of the testicular tissues in the observation groups 1, 2, 3, and the control group were 335.67 ±41.12,452.75 ±37.55,547.25 ±41.11,and 210.32 ±37.58, respectively; significant difference was found in ROS level between every two groups(all P<0.05).The protein expression of GRP78,CHOP,Caspase-12, and Caspase-3 in the offspring rat testicular tissues of the observation groups was higher than that of the control group(P<0.05).Compared with the observation group 1,the expression of these proteins in the observation groups 2 and 3 signifi-cantly increased(P<0.05);at the same time,the expression of these proteins in the observation group 3 was higher than that of the observation group 2(P<0.05).Conclusions After the long-term administration of manganese chloride in mother rats,the apoptosis level of spermatogenic cells increases in the offspring male rats.The underlying mechanism may be that maternal long-term MnCl2 exposure elevates ROS levels in testicular tissues of the offspring male rats which acti -vates CHOP/Caspase-2,an endoplasmic reticulum specific apoptotic signaling pathway,and ultimately leads to Caspase-3 activation.关键词
氯化锰/生精细胞/细胞凋亡/内质网应激/大鼠Key words
manganese chloride/spermatogenic cells/apoptosis/endoplasmic reticulum stress/rats分类
医药卫生引用本文复制引用
王乾兴,于明明,李姣,路健,汤贤春,张先平..长期氯化锰腹腔注射母鼠的子代雄鼠生精细胞凋亡变化及其机制探讨[J].山东医药,2018,58(3):23-26,4.基金项目
贵州省自然科学基金项目(黔科合J字20112282) （黔科合J字20112282）
