山东医药2018，Vol.58Issue(3)：31-33,3.DOI:10.3969/j.issn.1002-266X.2018.03.008
耐紫杉醇宫颈癌细胞株Hela特异性结合多肽的筛选及其功能鉴定
Screening and functional identification of peptides specifically binding to Paclitaxel-resistant cervical cancer cells Hela
摘要
Abstract
Objective To screen out the peptides specifically binding to Paclitaxel-resistant cervical cancer cells Hela (Hela/PTX).Methods Using drug-resistant cervical cancer Hela/PTX cells as the target cells,and the phage 7 peptide library was screened for 3 rounds by competitive binding tests.Using ELISA to determine the affinity of peptides to Hela/PTX cells(P/N value).Ten peptides with the high P/N value were selected to be sequenced,and we named a polypeptide with the highest P/N value and synthesized this peptide in vitro according to its sequence.The molecular weight of the pep-tide was determined by mass spectrometry.The affinity of the peptide to Hela/PTX and osteosarcoma cells was observed by cell affinity experiment.Results The selected peptide was named EP-7.Mass spectrometry analysis showed that the mo-lecular weight of EP-7 was 815.30D.The results of cell affinity test showed that there was green fluorescence in the cyto-plasm of Hela/PTX,but no green fluorescence was observed in osteosarcoma cells.Conclusion The peptide EP-7 which can bind specifically to Hela/PTX cells is successfully screened out.关键词
宫颈癌/耐药宫颈癌细胞/噬菌体肽库/多肽Key words
cervical carcinoma/drug-resistant cervical cancer cells/phage peptide library/polypeptide分类
医药卫生引用本文复制引用
李倩,左璇,邓成程,尹东锋..耐紫杉醇宫颈癌细胞株Hela特异性结合多肽的筛选及其功能鉴定[J].山东医药,2018,58(3):31-33,3.基金项目
国家自然科学基金资助项目(81372782). （81372782）
