森林工程2018，Vol.34Issue(2)：26-29,4.
树莓品种"波拉纳"组培繁殖体系建立
Establishment of Tissue Culture and Propagation System for Raspberry (Rubus idaeus) Cultivar "Polana"
摘要
Abstract
In order to explore the optimal tissue culture conditions for Raspberry (Rubus idaeus) Cultivar "Polana", which can produce a large scale of high quality seedlings by factory nusery, stem segments were selected as experiment materials, studying the sterilization methods of the explant, basal medium types, proliferation methods and rooting method by orthogonal test. The results showed that the optimal sterilization method of stem segments was 75% Ethanol for 30 s +0.1%HgCl2for 8 min + 2% NaClO for 2 min. The contamination percentage, browning percentage and survival percentage were 2.88%, 15.93% and 81.11% respectively. Stem segments could be germinated and expanded leaf on three kinds of media (WPM、1/2 MS、MS). But on the MS medium, the axillary bud was bright green and vigorous. Thus, the optimal primary culture medium was MS+6-BA0.5 mg·L-1+NAA0.1 mg·L-1. The optimal proliferation medium and hormone combination of plantlet was MS+KT1.0 mg·L-1+NAA0.1 mg·L-1+6-BA0.5 mg·L-1, multiplication index was 5.14 and the height of plantlet was 2.89 cm. On the 25thday, rooting percentage was 100%, the rooting number was up to 6.14 and the main root were strong.关键词
树莓/组织培养/植物生长调节剂/培养基Key words
Raspberry "Polana"/tissue culture/plant growth regulator/medium分类
农业科技引用本文复制引用
李艳霞,刘忠玲,刘建明,李桂君..树莓品种"波拉纳"组培繁殖体系建立[J].森林工程,2018,34(2):26-29,4.基金项目
黑龙江省森工总局项目(sgzjY2015024) （sgzjY2015024）
