兽类学报2017，Vol.37Issue(3)：293-299,7.DOI:10.16829/j.slxb.201703010
牦牛Endothelin-1基因CDS序列克隆及其真核表达质粒构建
Cloning of the CDS of yak Endothelin-1 gene and construction of its eukaryotic expression plasmid
摘要
Abstract
Endothelin-1 is a potent vasoconstricting factor that can induce structural and functional changes of the blood vessel by promoting vascular smooth muscle cell proliferation.This study was designed to study the molecular basis of highaltitude adaption in yak with a focus on the potential role of Endothelin-1.We cloned and analyzed the CDS of yak (Bos grunniens) Endothelin-1 and constructed a eukaryotic expression plasmid to provide a useful tool to study its fuction in the future.The results showed that the full-length of yak Endothelin-I contains a complete ORF (609 bp) encoding 202 amino acids.The putative molecular weight and theory isoelectric point of Endothelin-1 gene in yak are 22.98 kDa and 9.63.We also successfully constructed a eukaryotic expression vector pEGFP-C1-Endothelin-1 in which the CDS of the Endothelin-1 is driven by CMV promoter and the expression can be monitored by GFP.Amino acid sequences of yak Endothelin-1 are 100％,93％,81％,77％,70％ identical to those in Bos taurus,Ovis aries,Pantholops hodgsoni and Homo sapiens,respectively.In summary,findings from the study will pave the way to further explore the molecular mechanism of high-altitude adaption in yak and likely other animals.关键词
牦牛/内皮素1/分子克隆/真核表达载体Key words
Endothelin-1/Eukaryotic expression vector/Molecular cloning/Yak分类
生物科学引用本文复制引用
汪迁昊,刘健锋,丁艳平,王建林,邵宝平..牦牛Endothelin-1基因CDS序列克隆及其真核表达质粒构建[J].兽类学报,2017,37(3):293-299,7.基金项目
国家自然科学基金项目(31000190,051000006,31060141) （31000190,051000006,31060141）
