食品科学2018，Vol.39Issue(4)：176-187,12.DOI:10.7506/spkx1002-6630-201804027
超高效液相色谱-串联质谱法同时测定5个产地花椰菜和西兰花中的23种酚酸类化合物
Simultaneous Determination of Twenty-Three Phenolic Acids in Cauliflower(Brassica oleracea L.var.botrytis L.)and Broccoli(B.oleracea L.var.italica)from Five Producing Places by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method for the simultaneous determination of twenty-three phenolic acids in cauliflower(Brassica oleracea L.var.botrytis L.)and broccoli(B.oleracea L.var.italica)was developed by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The target analytes were separated on an Acquity UPLC HSS T3 column (2.1 mm × 150 mm, 1.8 μm) under a gradient elution mode using a mobile phase consisting of 0.1% formic acid and 0.1% formic acid acetonitrile. The flow rate was 0.3 mL/min and the column temperature was 45 ℃. The target compounds were analyzed in the negative ion multiple reaction monitoring (MRM) mode. Good linear relationships were obtained for all the analytes in the concentration range of 1.0–500.0 μg/L were obtained with high correlation coefficients over 0.992. The precision, repeatability and stability, expressed as relative standard deviation (RSD), were lower than 5.10%. The recoveries from spiked samples ranged from 81.32% to 100.39%, with RSD below 6.21%. The proposed method was applied to the determination of phenolic compounds in broccoli and cauliflower samples collected from five different cities. The method showed time saving, high sensitivity, and good repeatability, and was suitable for the simultaneous determination of phenolic compounds in cauliflower, broccoli and other vegetables.关键词
超高效液相色谱-串联质谱/酚酸/花椰菜/西兰花Key words
UPLC-MS/MS/phenolic acids/cauliflower(Brassica oleracea L.var.botrytis L.)/broccoli(B.oleracea L.var.italica)分类
化学化工引用本文复制引用
马帅,王纪华,高媛,王蒙,冯晓元..超高效液相色谱-串联质谱法同时测定5个产地花椰菜和西兰花中的23种酚酸类化合物[J].食品科学,2018,39(4):176-187,12.基金项目
国家农产品质量安全风险评估重大专项(GJFP201601503) （GJFP201601503）
公益性行业(农业)科研专项(201303075) （农业）
农业部农产品质量安全风险评估实验室(北京)开放课题(kfkt201701) （北京）
