生态学报2017，Vol.37Issue(19)：6616-6623,8.DOI:10.5846/stxb201607071390
镉对苯并(a)芘在蚯蚓亚细胞组分中分配积累的影响
Effects of cadmium on the distribution and accumulation of benzo [a] pyrene in subcellular fractions of Eisenia fetida
摘要
Abstract
This experiment explored the mechanism underlying benzo[a] pyrene (BaP) distribution in different subcellular fractions of the earthworm Eisenia fetida (Fraction C:associated with the cytosol;Fractions D:associated with granules;and Fraction E:associated with tissue fragments and cell membranes) under conditions of contamination with both BaP and cadmium ions (Cd2+) at different concentrations.The results showed that Cd2+ inhibited the accumulation of BaP in the earthworm,and that BaP was accumulated to the greatest extent in Fraction E,followed by Fraction C and Fraction D.With the addition of Cd2+,BaP concentrations in the three fractions initially decreased,but subsequently increased with the increasing concentration of Cd2+,whereas protein content and acetylcholinesterase (AChE) activity showed the opposite trend.In contrast,glutathione s-transferase (GST) activity initially decreased and then subsequently increased with increasing Cd2+ concentration in Fraction C and E,whereas the activity gradually increased in Fraction D.Correlation analysis indicated that protein content showed a significant negative correlation with BaP concentration in Fraction C and E;AChE activity showed a significant negative correlation with BaP concentration in Fraction C;and GST activity showed a non-significant correlation with BaP concentration.Collectively,the results indicate that BaP mainly accumulated in Fraction E,and that the accumulation of BaP in Fraction C and E was correlated with the content of protein and activity of AChE,which was influenced by Cd2+.Furthermore,BaP concentration initially decreased,but subsequently increased,with the increasing concentration of Cd2+.关键词
苯并(a)芘/镉/赤子爱胜蚓(Eisenia fetida)/亚细胞组分/复合污染Key words
benzo[a] pyrene/cadmium/Eisenia fetida/subcellular fraction/combined pollution引用本文复制引用
周丽娜,周静,李辉信,胡锋,徐莉..镉对苯并(a)芘在蚯蚓亚细胞组分中分配积累的影响[J].生态学报,2017,37(19):6616-6623,8.基金项目
国家自然科学基金(41371469) （41371469）
公益性行业(农业)科研专项经费(201503121) （农业）
江苏省优势学科项目资助 （）
