深圳大学学报（理工版）2018，Vol.35Issue(2)：172-178,7.DOI:10.3724/SP.J.1249.2018.02172
莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析
Bioinformatical analysis of genes encoding APC/C complex and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii
摘要
Abstract
Chlamydomanos reinhardtii is a model microalgae for lipids production. There is a contradiction between lipids biosynthesis and biomass accumulation in C. reinhardtii. Revealing regulation mechanisms of cell cycle can prove a new direction for promoting biomass in C. reinhardtii. In this paper, the gene structures, protein characteristics,and phylogenetic tree of anaphase-promoting complex/cyclosome (APC/C) gene family involving in cell cycle control are analyzed through bioinformatics. Totally 13 genes of APC/C family are identified in C. reinhardtii,and one gene cluster is found in chromosome 13. The phylogenetic tree shows that APC/C proteios, except the activation factors CrCDC20 and CrCDH1 from C. reinhardtii,have low homologous with the proteios in higher plants. Meanwhile,the gene expression profiles of CrCDC20 under sulfur or nitrogen deficiency conditions are determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction (rt-RT-qPCR). The results show that CrCDC20 is up-regulate under sulfur deficiency but down-regulate under nitrogen deficiency, which indicates that CrCDC20 might involve in nutrition deficiency stresses.关键词
微藻分子生物学/生物柴油/细胞周期/E3泛素连接酶/进化树分析/实时荧光定量反转录聚合酶链式反应Key words
molecular biology of microalgae/biodiesel/cell cycle/E3 ubiquitin ligase/phylogenetic analysis/real-time fluorescence quantitative reverse transcription polymerase chain reaction (rt-RT-qPCR)分类
农业科技引用本文复制引用
罗秋兰,王潮岗,胡章立..莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析[J].深圳大学学报（理工版）,2018,35(2):172-178,7.基金项目
国家自然科学基金资助项目(31700309,31470389,31470431) （31700309,31470389,31470431）
广东省自然科学基金资助项目(2014A030308017,2017A030310255) （2014A030308017,2017A030310255）
深圳市科技计划资助项目(JCYJ20150324141711583)National Natural Science Foundation of China(31700309,31470389,31470431) （JCYJ20150324141711583）
Natural Science Foundation of Guangdong Province(2014A030308017,2017A030310255) （2014A030308017,2017A030310255）
Shenzhen Science and Technology Research Foundation(JCYJ20150324141711583) （JCYJ20150324141711583）
