天然产物研究与开发2018，Vol.30Issue(1)：15-20,6.DOI:10.16333/j.1001-6880.2018.1.003
恰麻古多糖对巨噬细胞RAW264.7体外免疫调节作用初探
Immunomodulatory Effects of Brasssica rapa L.Polysaccharides on Macrophage RAW264.7 in vivo
摘要
Abstract
The aim of this study is to discuss the immune regularity effects of Brassica rapa L.polysaccharide (BRP),Brassica rapa L.natural polysaccharides (BRNP-1,BRNP-2) and Brassica rapa L.acidic polysaccharides (BRAP-1,BRAP-2) on macrophage RAW264.7.CCK-8 kit was used to detect the proliferation rate of different concentrations of polysaccharides.The phagocytosis activity was measured by toluylene red.Griess reagent was used to detect the release of NO level.ELISA kit was used to detect the secretion level of TNF-α and IL-6.The results showed that different concentrations of polysaccharides could significantly improve the proliferation rate and phagocytosis activity of macrophageR-AW264.7,and it could also stimulate the RAW264.7 releasing NO,and promote the secretion of TNF-α and IL-6.This study preliminary verifies that the polysaccharides have good immune adjustment activity on macrophageRAW264.7.关键词
恰麻古/多糖/巨噬细胞RAW264.7/免疫调节Key words
Brasssica rapa L./polysaccharides/macrophage RAW264.7/immunomodulatory分类
医药卫生引用本文复制引用
热孜亚木·吾甫尔,李月红,海力茜·陶尔大洪..恰麻古多糖对巨噬细胞RAW264.7体外免疫调节作用初探[J].天然产物研究与开发,2018,30(1):15-20,6.基金项目
国家自然科学基金(81460615) （81460615）
