天然产物研究与开发2018，Vol.30Issue(1)：21-26,6.DOI:10.16333/j.1001-6880.2018.1.004
金线莲多糖对免疫抑制小鼠脾淋巴细胞体外增殖、分泌NO及细胞因子的影响
Effects of Anoectochilus roxburghii Polysaccharide on Splenic Lymphocytes Proliferation, NO and Cytokine Secretion in Immunosuppressed Mice in vitro
摘要
Abstract
This study was designed to elucidate the effects of Anoectochilus roxburghii polysaccharide (ARP) on splenic lymphocytes proliferation,NO and IL-2,IL-6,IFN-γsecretion in immunosuppression cyclophosphamide-induced mice in vitro.Splenic lymphocytes proliferation were measured by MTT assay,the concentration of NO in supernatant was determined by Griess reaction and content of IL-2,IL-6 and IFN-γ in the supernatant was detected by Enzyme-linked immunosorbent assay (ELISA).The results showed compared with control group,ARP (50-400 μg/mL) possessed the stimulation effect on murine splenic lymphocytes (P ＜0.01) in a dose-dependent manner,the production of NO and the level of IL-2,IL-6 and IFN-γ were increased (P ＜ 0.05,P ＜ 0.01).It was concluded that ARP can improve the immune activity in immunosuppressed mice in vitro via promoting splenocytes proliferation and increasing the secretion of NO and cytokines of IL-2,IL-6,IFN-γ.关键词
金线莲多糖/免疫抑制小鼠/脾淋巴细胞增殖/NO/细胞因子Key words
Anoectochilus formosanus polysaccharide/immunosuppressed mice/splenic lymphocytes proliferation/NO/cytokines分类
农业科技引用本文复制引用
马玉芳,郑小香,衣伟萌,任喆,李健,秦韬,黄一帆..金线莲多糖对免疫抑制小鼠脾淋巴细胞体外增殖、分泌NO及细胞因子的影响[J].天然产物研究与开发,2018,30(1):21-26,6.基金项目
国家自然科学基金(31372474) （31372474）
