天然产物研究与开发2018，Vol.30Issue(1)：27-32,160,7.DOI:10.16333/j.1001-6880.2018.1.005
沙棘黄酮制备及体外抑制人前列腺癌PC-3细胞作用研究
Preparation of Flavonoids From Sea buckthorn and Its Inhibitory Effect on Human Prostate Cancer PC-3 cells in vitro
摘要
Abstract
Using AB-8 macroporous resin as carrier and gradiently eluted by 10％,30％,50％ ethanol,the purified samples were screened with the content of total flavonoids and flavonoid aglycones.The MTT assay and RTCA were used to examine inhibitory effect of flavonoids from Sea buckthorn on PC-3 cells.Flow-cytometry was used to analyze the cell apoptosis and cell cycle of the PC-3 cells.The expressions level of Bax and Bcl-2 were detected by Western blot.The results showed that in the gradient elution of 50％ ethanol solution (S50),the content of total flavonoids was 25.42％,hydrolyzing it to get SH50 sample,which quercetin and isorhamnetin content reached 5.03％ and 18.64％ respectively;SH50 (25 μg/mL) can inhibit the activity of PC-3 cells and showed the feature of time/dose dependent;25 μg/mL SH50 can also induce the apoptosis of PC-3 cells significantly (P ＜ 0.01) and block the cell cycle at G2/M stage after treated 48h.Moreover,SH50 can increase the activities of Bax but decrease the expression level of Bcl-2.These results demonstrated that SH50 can inhibit the proliferation and induce cell apoptosis of PC-3 cells in vitro,the mechanism might be related with the blocking of the cell cycle and regulating the expression levels of Bax and Bcl-2.关键词
沙棘黄酮/人前列腺癌/PC-3细胞/抑制增殖Key words
sea buckthorn flavonoids/human prostate cancer/PC-3 cells/inhibit the proliferation分类
医药卫生引用本文复制引用
赵波,向晓玲,王微,李春松,吴晓琴,沈建福..沙棘黄酮制备及体外抑制人前列腺癌PC-3细胞作用研究[J].天然产物研究与开发,2018,30(1):27-32,160,7.基金项目
国家自然科学基金面上项目(J20120653) （J20120653）
