天然产物研究与开发2018，Vol.30Issue(1)：45-51,96,8.DOI:10.16333/j.1001-6880.2018.1.008
豆腐柴叶挥发油化学成分及其抗氧化和抑菌作用研究
Chemical Constituents, Antioxidant and Antimicrobial Effects of Volatile Oil from Premna microphylla Leaves
摘要
Abstract
The volatile oil from Premna microphylla leaves was extracted by water-steam distillation and analyzed by GC-MS.The antioxidant activity of volatile oil was examined using various antioxidant assays,such as reducing power,1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) free radical scavenging assays.The volatile oil was also evaluated for antimicrobial activity by paper disc diffusion method.The results showed that twenty-six compounds representing 98.10％ of the total constituents were identified.The major constituents of the volatile oil were propanoic acid ethyl ester (33.70％),2,2'-methylenebis (6-tert-butyl-4-methylphenol)(9.38％),phytol (7.91％),c-eudesmol (3.75％),squalene (3.39％),and palmitic acid (3.34％).The volatile oil exhibited strong antioxidant capacities such as reducing power,DPPH and ABTS free radical scavenging abilities.When the scavenging rate against DPPH and ABTS free radicals was 50％,the concentrations of volatile oil were 3.396 mg/mL and 0.761 mg/mL,respectively.The volatile oil also had the inhibition effects on E.coli,S.aureus,B.subtilis and P.aeruginosa in a dose-dependent manner.Its minimum inhibitory concentration (MIC) against four common bacterial strains was 1.125 mg/mL.In conclusion,the volatile oil from P.microphylla leaves showed remarkable antioxidant and antimicrobial activities,and this research provided theoretical basis for the further study and application of volatile oil in food and medicine products.关键词
豆腐柴叶/挥发油/化学成分/抗氧化/抑菌Key words
Premna microphylla leaves/volatile oil/chemical constituents/antioxidant/antimicrobial分类
医药卫生引用本文复制引用
吴永祥,杨庆,李林,卞国勇,胡高峰,毕淑峰..豆腐柴叶挥发油化学成分及其抗氧化和抑菌作用研究[J].天然产物研究与开发,2018,30(1):45-51,96,8.基金项目
安徽省高校自然科学研究重点项目(KJ2017A398) （KJ2017A398）
安徽省留学回国人员创新项目择优资助计划重点项目(2017srst1) （2017srst1）
博士人才引进启动基金(2016xkj q004) （2016xkj q004）
安徽省大学生创新创业训练计划(AH201610375055) （AH201610375055）
