天然产物研究与开发2018，Vol.30Issue(1)：56-60,154,6.DOI:10.16333/j.1001-6880.2018.1.010
黄芩饮片、标准汤剂、中间体、配方颗粒的HPLC指纹图谱相关性研究
Correlation Analysis of HPLC fingerprint of Pieces-standard Decoction-Intermediates and Dispensing Granules of Scutellaria baicalensis
陈玲 1李晓 1魏悦 1曹静亚 1范毅1
作者信息
- 1. 河南省生物技术开发中心河南省植物天然产物开发工程技术研究中心河南科高中标检测技术有限公司,郑州450002
- 折叠
摘要
Abstract
In this study,the HPLC fingerprint method of the pieces,standard decoction,intermediates and dispensing granules of Scutellaria baicalensis was established,and the correlations among them were evaluated.The chromatographic separation was carried out on an Agilent Eclipse XDB C18 column (250 mm ×4.6 mm,5 μm);The mobile phase was eluted with gradient by methanol-0.05 ％ phosphoric acid;The flow rate was 1.0 mL/min.The column temperature was 35 ℃ and the detection wavelength was set at 275 nm.There were 9 common characteristic peaks in all fingerprint spectrum of 10 batches of pieces,standard decoction,intermediates and dispensing granules of S.baicalensis,presenting a good correlation,in the 9 common characteristic peaks,three of them were baicalin,baicalein,wogonin.The main chemical constituents of S.baicalensis pieces,standard decoction,intermediates and dispensing granules composition are basically identical.The established HPLC fingerprint method can be used for the quality control of S.baicalensis dispensing granules.关键词
黄芩/指纹图谱/相关性/配方颗粒/标准汤剂Key words
Scutellaria baicalensis/fingerprint/correlation/dispensing granules/standard decoction分类
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陈玲,李晓,魏悦,曹静亚,范毅..黄芩饮片、标准汤剂、中间体、配方颗粒的HPLC指纹图谱相关性研究[J].天然产物研究与开发,2018,30(1):56-60,154,6.
