天然产物研究与开发2018，Vol.30Issue(1)：73-78,6.DOI:10.16333/j.1001-6880.2018.1.013
基于“碳源”研究参苓白术散中药多糖对二株肠道拟杆菌体外生长的影响
Study on the in vitro Growths of Two Typical Intestinal Bacteroides under Influence of Shenlingbaizhu Powder Polysaccharide as "Carbon Source"
摘要
Abstract
Shenlingbaizhu powder is a classical prescription with the function of tonifying spleen.Modem researches have found that its clinical efficacy is closely related to the regulation of intestinal flora;however,it is not clear which ingredient works and which bacteria are regulated.Since polysaccharide is a main ingredient of traditional Chinese medicine,and Bacteroides is the most abundant bacterial group which occupied the advantages in the utilization of polysaccharides,we propsoed that Shenlingbaizhu powder polysaccharide may play roles in regulating the intestinal flora (Bacteroides as the most significant target) as bacterial competitive carbon source.In this study,we explored the in vitro growth of two typical Bacteroides under the influence of Shenlingbaizhu powder polysaccharide as "carbon source".Two typical Bacteroides strains were cultured under different carbon sources (1-40％ concentration of traditional Chinese medicine polysaccharides,glucose,BHI),with two Firmicutes as control.The in vitro growth rates on different carbon sources were determined by continuous samplings.The results showed that the in vitro growths of Bacteroides were influenced by Shenlingbaizhu powder polysaccharide under different concentrations (the optimal concentration of polysaccharide is 20％).As for the in vitro growth of Bacteroides thetaiotaomicron ATCC29148 under different carbon sources on,the result as below:BHI group ＞ the optimal concentration of polysaccharide group ＞ Glucose group,while that of Bacteroides fragile ATCC25285 is:the optimal concentration of polysaccharide ＞ BHI group ＞ Glucose group.As for the in vitro growth of different bacteria under influences of Shenlingbaizhu powder polysaccharide,the result is:Bacteroides ＞ Firmicutes.In conclusion,the traditional Chinese medicine polysaccharide can be used regulate the balance of intestinal flora as carbon prebiotics for Bacteriodetes,which may be one of the micro ecological mechanisms related to the clinical effect of Shenlingbaizhu powder.关键词
参苓白术散/多糖/拟杆菌/厚壁菌/碳源Key words
Shenlingbaizhu powder/polysaccharide/Bacteroides/Firmicutes/carbon source分类
医药卫生引用本文复制引用
张丽萍,伍荷洁,舒青龙..基于“碳源”研究参苓白术散中药多糖对二株肠道拟杆菌体外生长的影响[J].天然产物研究与开发,2018,30(1):73-78,6.基金项目
国家自然科学基金(81473455,31560038) （81473455,31560038）
江西省自然科学基金(20171BAB205087) （20171BAB205087）
