复方龙脉宁不同提取液对H2O2诱导H9c2心肌细胞氧化损伤的保护作用OA北大核心CSCDCSTPCD
Protective Effect of Compound Longmaining Extracts on H2O2-Induced H9c2 Cardiomyocytes Injury
采用不同浓度H2 O2处理H9c2心肌细胞,MTT法检测复方龙脉宁(Compound Longmaining,CLMN)水提液和醇提液含药血清(5％、10％、20％组)对氧化损伤的H9c2心肌细胞活性的影响,研究CLMN提取液对H2 O2导致H9c2心肌细胞氧化损伤的修复作用.结果显示,当H2 O2浓度为100μM,作用时间24h,可建立稳定的H9c2心肌细胞氧化损伤模型;与对照组相比,经H2 O2处理后的细胞活性明显下降,SOD活性显著降低,而…查看全部>>
H9c2 cardiomyocytes were treated with different concentrations of H2 02.The effect of Compound Longmaining (CLMN) water extract and alcohol extract (5％,10％,20％ group) on H9c2 cardiomyocytes injured by H2 O2was detected by MTT assay.The results showed that a stable H9c2 cardiomyocytes injury model can be established when the concentration of H2O2 was 100 μM and the action time was 24 h.Compared with the control group,the activity of the cells decreased obviou…查看全部>>
宋逍;王昌利;段玺;张文娟;董林娟
陕西中医药大学药学院,咸阳712046陕西中医药大学药学院,咸阳712046陕西中医药大学附属医院检验科,咸阳712046陕西中医药大学药学院,咸阳712046陕西中医药大学药学院,咸阳712046
医药卫生
复方龙脉宁提取液H9c2心肌细胞MTT法心肌氧化损伤
CLMN extractH9c2 cardiomyocytesMTT methodmyocardial oxidative injury
《天然产物研究与开发》 2018 (1)
皂苷类提取物生物药剂学特征与其物理化学性质的相关性研究
79-83,5
国家自然科学基金资助(81373944,81373978)陕西省科学技术厅课题(2012KTCQ03-13)陕西省中药制药重点学科专项
评论