现代妇产科进展2018，Vol.27Issue(3)：165-169,5.DOI:10.13283/j.cnki.xdfckjz.2018.03.002
C/EBPα在妊娠期糖尿病大鼠胎肺成熟过程中的作用
The effect of CCAAT enhancer binding proteinαon lung maturation of the fetuses from STZ-induced diabetic pregnancy rats
摘要
Abstract
Objective:To investigate the effect of CCAAT enhancer binding protein αon lung maturation of the fetuses from STZ-induced diabetic pregnancy rats. Methods:Pregnant Sprague-Dawley rats were randomly divided into diabetic and control group. Diabetic pregnancy was induced by a single intraperitional injection of streptozotozin. The blood glucose and weight of the pregnant rats were monitored every other day during the gestation. Fetuses were delivered successively by cesarean section on day 21 of pregnancy. The blood glucose,body weight and litter size were recorded. The morphological alterations of fetal lungs were observed by using HE staining. The lipid accumulation in fetal lungs was evaluated by Oil red O staining. The ultra-structures of the fetal lungs were observed by transmission electron microscope. The expressions of SP-A and C/EBPαwere detected by immunohistochemistry. The expressions of SP-A and C/EBP α gene were analyzed by RT-PCR. The expressions of SP-A and C/EBP α protein were measured by Western blot. Results:Blood glucose level of female rats and fetal rats in GDM were significantly higher than those of the control group (P<0. 000),while body weights of the pregnant rats and fetal rats in GDM group were lower than that in the control group ( P<0 . 000 ) . The Litter size in GDM was significantly reduced compared with the control group ( P<0 . 000 ) . The mean alveolar numbers and pulmonary alveolar area of GDM group were signifi-cantly lower than those of the control group ( P<0 . 000 ) . The width of alveolar septa in GDM group was thicker than that in the control group (P<0. 000). In GDM group,the volume of the AEC Ⅱ cells ( the alveolar typeⅡcells ) were significantly smaller than control group,and the shape of AEC Ⅱ cells became flat and accompanied with decreased lamellar body, abundant glycogen accumulated in the cytoplasm. The microvilli on the surface of the alveolar type Ⅱcells were obviously reduced. The mRNA and protein expressions of SP-A and C/EBPαof fetal lung in GDM group were significantly decreased compared with that in the control group ( P<0 . 05 ) . Conclusions:The decreased expression of C/EBP α in GDM fetal lung inhibited the production of SP-A and lipid,which prevents intracellular glycogen from transforming into lip-ids. The decreased expression of C/EBP in fetal lung tissue may be one of the pathogenesis of delayed development of GDM fetal lung.关键词
CCAAT/增强结合蛋白α/妊娠期糖尿病/胎肺成熟Key words
CCAAT enhancer binding protein α/Gestational diabetes mellitus/Fetal lung maturation分类
医药卫生引用本文复制引用
李金艳,杨根岭..C/EBPα在妊娠期糖尿病大鼠胎肺成熟过程中的作用[J].现代妇产科进展,2018,27(3):165-169,5.基金项目
重庆市社会民生科技创新专项(No:cstc2015shmszxsydw-6) （No:cstc2015shmszxsydw-6）
