现代妇产科进展2018，Vol.27Issue(3)：170-174,178,6.DOI:10.13283/j.cnki.xdfckjz.2018.03.003
骨髓间充质干细胞来源的Exosome修复兔受损子宫内膜的研究
Bone marrow mesenchymal stem cell-derived Exosome repair damaged rabbit endome-trium
摘要
Abstract
Objective:To investigate the effect and mechanism of Exosome ( Exo) de-rived from bone marrow mesenchymal stem cells ( BMSCs) on endometrium injury repairmen. Methods:A total of 45 female rabbits were randomly diveded into control group(n=15),mod-eling group (n=15) and Exo treatment group(n=15). After mechanical and infectious double injuries,retention of lipopolysaccharide cotton threads for 2d were implemented to cause endo-metrium injury before uterine curettage. Only laparotomy was applied to control group instead of injuries. Exo suspension was injected to uterine intramural spaces 1 week after model establish-ment,PBS of the same volume was injected to the other two groups. Uterine tisues 1w,2w and 4 w after treatment were respectively collected for HE and Masson staining in order to calculate the gland numbers and fibrosis area of endometrium. Immunohistochemical ( IHC) staining were adopted for the detection of Cytokeratin-19 ( CK-19 ) and Vimentin ( Vim ) expressions. Re-sults:After 4w of treatment,the gland number of Exo group (26. 17±1. 254) was higher than that of modeling group(10. 92±0. 848)(P<0. 05),which the fibrosis area of Exo group was lower than modeling group ( 49 . 25 ± 1 . 239 ) with statistical significance ( P<0 . 05 ) . The ex-pression of CK-19 protein was significantly higher than that in the modeling group ( P<0 . 05 ) , the expression of VIM protein was significantly lower than that in the modeling group ( P<0 . 05 ) and no significant difference was discovered in each index compared with the control group ( P>0 . 05 ) . Conclusion:Exo derived from BMSCs is able to promote gland hyperplasia in injured endometrium,to inhibit the development of fibrosis,and to improve the endometrium re-pairment.关键词
宫腔粘连/间充质干细胞/外泌体/细胞治疗Key words
Intrauterine adhesion/Mesenchymal stem cell/Exosome/Transplant分类
医药卫生引用本文复制引用
王雪,刘芳..骨髓间充质干细胞来源的Exosome修复兔受损子宫内膜的研究[J].现代妇产科进展,2018,27(3):170-174,178,6.基金项目
国家自然科学基金地区项目(No:81460225) （No:81460225）
