现代妇产科进展2018，Vol.27Issue(3)：191-194,4.DOI:10.13283/j.cnki.xdfckjz.2018.03.008
miR-186通过下调EGFR表达对宫颈癌细胞增殖与侵袭的影响
miR-186 down-regulate EGFR expression and affect on proliferation and invasion of cervi-cal cancer
魏莉 1崔立新 1赵兴波2
作者信息
- 1. 德州市立医院妇科,德州 253012
- 2. 山东省立医院妇科,济南 250021
- 折叠
摘要
Abstract
Objective:To investigate the expression of miR-186 in cervical cancer tissues and cells,and to discuss its mechanism implicated in the development of cervical cancer. Meth-ods:The expression levels of miR-186 and EGFR were detected by RT-PCR and Western blot in 30 samples of cervical cancer tissue and 30 samples of normal cervical tissues. The relationships between miR-186 expression and clinicopathological features of cervical cancer were analyzed. CCK-8 assay was used to analyze the effects of miR-186 on the proliferation and invasion of cervi-cal cancer cells SiHa and HeLa. Luciferase assays were used to determined whether EGFR-3'UTR was the direct target of miR-186. Results:miR-186 expression in the cervical cancer tissues was significantly lower than that in the normal cervical tissues, while the expression of EGFR was higher than that in the normal cervical tissues. The expression miR-186 was inversely related to the expression of EGFR( EGFR mRNA:R2=0. 865,P<0. 001;EGFR protein:R2=0. 832,P<0. 001). The expression level of miR-186 in the cervical cancer tissues was significantly correlated with degree of differentiation and lymphatic metastasis up-regulated miR-186 expression would significantly reduce the proliferation and invasion of SiHa and HeLa. Luciferase assays showed that miR-186 binds the EGFR-3'UTR and affect the expression of EGFR protein. EGFR over-ex-pression reversed miR-186-inhibited cell proliferation and invasion. Conclusions:miR-186 ex-pression can inhibit the proliferation and invasion of cervical cancer SiHa and HeLa cells through targeting EGFR-3'UTR.关键词
miR-186/表皮生长因子受体/宫颈癌Key words
miR-186/EGFR/Cervical cancer分类
医药卫生引用本文复制引用
魏莉,崔立新,赵兴波..miR-186通过下调EGFR表达对宫颈癌细胞增殖与侵袭的影响[J].现代妇产科进展,2018,27(3):191-194,4.
