新疆农业科学2018，Vol.55Issue(1)：1-8,8.DOI:10.6048/j.issn.1001-4330.2018.01.001
棉花GhFAD2-1基因5'UTR内含子的克隆与序列分析
Cloning and Sequence Analysis of 5'UTR Intron of GhFAD2-1 Gene from Cotton(Gossypium hirsutum L.)
摘要
Abstract
[Objective] Cloning and sequence analysis of the 5'UTR intron of the delta 12-oleic acid desaturase gene GhFAD2-1 would lay the foundation for the study of the expression and regulation of GhFAD2-1.[Method]In this study,the 5'UTR sequence of GhFAD2-1 was cloned by 5'RACE technology.And then,5'UTR intron of GhFAD2-1 was cloned in combination with the cotton genome sequence.Cis elements were also analyzed by PLACE and other bioinformatics software.[Result]The GhFAD2-1 5'UTR sequence 77 bp was obtained from Gossypium hirsutum using 5'RACE technique.The result showed that the transcription start site is T based on a combination of 5'UTR and genome sequence analysis.GhFAD2-1 5'UTR contained a full-length 1,103 bp intron sequence in the A genome of cotton.The full-length sequence of GhFAD2-1 5'UTR intron was 1,111 bp in D genome.The cleavage sites of 5'UTR intron were AA-GG, CA-GC, re-spectively.Cis element analysis showed that the intron included some typical function and optical response re -lated elements and elements related to hormone regulation or stress response.[Conclusion]5'UTR intron se-quences of GhFAD2-1 gene were cloned from the A and D genome,respectively.The transcription start site and the cleavage sites of 5'UTR intron were also identified.The results have laid a foundation to further study expression and regulation of GhFAD2-1 at the molecular level.关键词
棉花/GhFAD2-1基因/5'UTR内含子/克隆/序列分析Key words
cotton/GhFAD2-1 gene/5'UTR intron/cloning/sequence analysis分类
农业科技引用本文复制引用
孙亮,文凤,刘峰,张新宇,孙杰..棉花GhFAD2-1基因5'UTR内含子的克隆与序列分析[J].新疆农业科学,2018,55(1):1-8,8.基金项目
国家自然科学基金项目"棉花FAD2-1基因的5'UTR内含子与启动子的顺式作用元件鉴定及转录调控作用研究"(31460361) （31460361）
国家自然科学基金项目(31460361,31101094) （31460361,31101094）
石河子大学育种专项(YZZX201601,gxjs2014-yz08)The National Natural Science Foundation of China＂Identification of the cis－regulatory elements in 5＇UTR intron and promoter of FAD2－1 gene of cotton and studies on transcriptional regulation mechanism＂(31460361)and Breeding Special Fund of Shehezi University(YZZX201601,gxjs2014－yz08) （YZZX201601,gxjs2014-yz08）
