新疆农业科学2018，Vol.55Issue(1)：16-23,8.DOI:10.6048/j.issn.1001-4330.2018.01.003
棉花秸秆纤维素降解菌的筛选鉴定与降解棉秆效果研究
Screening and Identification of Cellulolytic Strains from Cotton Straw and Its Effect on Degradation of Cotton Stalk
摘要
Abstract
[Objective] This study aims to screen and identify fungi which adhere on the surface of the cotton by using microbiology and molecular biology methods in order to obtain fungi with high cellulose decom-position ability.[Method]The cotton straw was inoculated into different selective media, and the larger hy-drolysis circle of strains was separated and selected.At the same time the strains'filter paper enzyme activity (FPA)and sodium carboxymethyl cellulose(CMC-Na)saccharification vitality were determined, and be-sides,the weight loss rate of cotton stalk fiber was observed continuously for 25 days.[Result]The diameter of the hydrolysis ring of the 4 main strains was SN-1 >SN-4 >SN-3 >SN-4(P =0.013).In terms of CMC -Na saccharification vitality,SN -1 was significantly lower than other three strains(P <0.05)at 24-96 h,and there was no significant difference between different strains of treatments(P >0.05)at 120 h.In terms of FPA activity,SN and SN 1-4 strains was significantly higher than SN -2 and SN -3 strains (P <0.05)at 24 and 120 h,at other time points,the strains were sorted according to the activity level:SN-1,SN-4 >SN-3 >SN-2(P <0.05).There were 4 bacterial strains obtained by using the method of microscopic and molecular identification,SN -1 was Aspergillus nige r,SN -2 was Gibberella monilifor-m is,SN -3 was Alternaria nees and SN -4 was Penicillium italicum.[Conclusion]The effect of Aspergil-lus niger on the degradation of cotton straw was the most remarkable.The activity of CMC-Na saccharifying enzyme,the activity of filter paper enzyme and the actual weight loss rate (degradation rate)of cotton straw were the best when inoculated with Aspergillus niger or Penicillium italicum.关键词
棉花/真菌/酶活性/降解效果Key words
cotton/fungi/enzymes/degradation effect分类
农业科技引用本文复制引用
孙美娜,张凡凡,王永强,魏晓燕,鲁建江..棉花秸秆纤维素降解菌的筛选鉴定与降解棉秆效果研究[J].新疆农业科学,2018,55(1):16-23,8.基金项目
兵团科技援疆项目(2013AB008) （2013AB008）
新疆维吾尔自治区研究生科研创新项目(XJGR2015037)Scientific and Technological Assistance to Xinjiang Program of XPCC(2013AB008),Innovation Projects of Graduate Students of Xinjiang Uygur Autonomous Region(XJGR2015037) （XJGR2015037）
