新疆农业科学2018，Vol.55Issue(1)：66-73,8.DOI:10.6048/j.issn.1001-4330.2018.01.008
盐胁迫对葡萄苗期多胺和激素代谢的影响
Effects of Salt Stress on Polyamine and Hormone Metabolism in Grape Seedling Stage
摘要
Abstract
[Objective] To study the dynamic changes of polyamine content, polyamine oxidase, poly-amine synthase activity and hormones in grape leaves under salt stress,and explore the effects of different salt stress intensity on the metabolism of polyamines and hormones.[Method]The pot culture was used to simulate the salt stress environment,and 4 NaCl treatment gradients were set up:0 g/kg(CK),1 g/kg,2 g/kg,3 g/kg.Polyamine content and hormone content were determined by high performance liquid chromatography (HPLC).Polyamine synthesis and decomposition enzyme activity were determined by ultraviolet spectropho -tometry.[Result]After salt stress treatment,polyamine content in grape leaves and abscisic acid content sig-nificantly increased compared with the control and zeatin content decreased significantly with the prolonging of stress time.Activities of Put,Spm,Spd,ABA,PAO,DAO,ADC,ODC,SAMDC increased first and then declined,reaching the peak in 10 days or after 10 days.ZT gradually decreased,and decreased with the in-crease of stress intensity.After significant analysis,leaf polyamine content and Put,Spm, Spd content were positively correlated.The activities of DAO,ADC,ODC were significantly correlated with Put content and the activity of SAMDC was positively correlated with the contents of Spm and PAO.[Conclusion]Metabolism of three polyamines by enzymes and hormones can alleviate salt stress in grape seedling stage,during which the main action is the polyamine synthase.The relationship between the contents of polyamine and hormone under salt stress is also very close.关键词
盐胁迫/葡萄/多胺/激素/PAO/DAO/ADC/ODC/SAMDCKey words
salt stress/grape/polyamines/hormones/PAO/DAO/ADC/ODC/SAMDC分类
农业科技引用本文复制引用
孙琳琳,郁松林,赵丰云,杨湘,杨夕..盐胁迫对葡萄苗期多胺和激素代谢的影响[J].新疆农业科学,2018,55(1):66-73,8.基金项目
国家自然科学基金项目"滴灌条件下不同水氮处理对酿酒葡萄年际根系形态发育和氮素代谢的影响"(31360464)The National Natural Science Foundation of China＂Effects of different water and nitrogen treatments on annual root morphological development and nitrogen metabolism of wine grape under drip irrigation＂(31360464) （31360464）
