新疆农业科学2018，Vol.55Issue(1)：116-122,7.DOI:10.6048/j.issn.1001-4330.2018.01.013
不同产地库尔勒香梨萼端黑斑病病原菌的分离鉴定
The Separation and Identification of the Pathogenic Bacteria of Korla Pear Calyx End Black Spot Disease in Different Growing Areas
摘要
Abstract
[Objective] To explore whether the black spot of Korla pear in Aksu and Bazhou were caused by the same pathogenic bacteria,which might lay a foundation for the prevention and control of this disease in the future.The article used the calyx end black spot Korla pear in the Aksu Area area of aksu and bazhou Ba-zhou Area as testing material to separate and purify pathogenic bacteria from the diseased fruit to study the pathogenic types of black spot of calyx.[Method]The mycelium was cultured with the method of underlined culture for morphological identification and biological identification of calyx end black spot disease was carried out through genomic DNA extraction, PCR amplification of 18S conserved sequence and 3,730 sequencing.[Result] Which was determined whether was a single fungus or mixed bacteria,and to makeidentified as mor-phological and molecular biological identification of the dominant or a dominant pathogenic microflora in terms of morphology and molecular biology.This result provided the theoretical basis for the prevention and control of the disease.[Conclusion]The results showed that the pathogenic bacteria of Korla pear calyx end black spot disease in the two growth areas of were monobacteria and were consistent with Alternaria alternate(Fr.) Keissl.关键词
库尔勒香梨/萼端黑斑病/病原菌/鉴定Key words
Korla pear/calyx end black spot/pathogenic bacteria/appraisal分类
农业科技引用本文复制引用
吴玉鹏,赵晓梅..不同产地库尔勒香梨萼端黑斑病病原菌的分离鉴定[J].新疆农业科学,2018,55(1):116-122,7.基金项目
新疆自然科学基金面上项目"新疆库尔勒香梨萼端黑斑病调查及病原菌生物学特性的研究"(2015211A030)The General Programs of Xinjiang Uygur Autonomous Region Natural Science Foundation"Investigation and Study on Biological Characteristics of Pathogenic Bacteria of Korla Pear Calyx End Black Spot Disease"(2015211A030) （2015211A030）
