苯扎溴铵和西酞普兰对小鼠角膜上皮和角膜全层厚度的影响
Effects of benzalkonium bromide and citalopram on the thickness of corneal epithelium and full-thickness of the cornea in mice
摘要
Abstract
Objective To explore the effects of benzalkonium bromide and citalopram on the corneal epithelium and corneal thickness of mice using optical coherence tomography angiography (OCTA).Methods Together 60 mice were randomly divided into 5 groups (group A,B,C,D and E;n =12),with group A left untreated,group B receiving PBS eye drops,group C given benzalkonium bromide eye drops,group D undergoing intraperitoneal administration of citalopram suspension,and group E treated with combination of benzalkonium bromide eye drops and citalopram suspension.After 2 weeks,OCTA was applied for corneal subarea,followed by measurement of the thickness of corneal epithelium and full-thickness of the cornea of all mice,and then the mean values were calculated.Results The thickness of corneal epithelium and fullthickness of the cornea was (66 ±7) μm and (141 ± 11) μm in the group A,(66 ± 8) μm and (140 ± 12) μm in the group B and D,(73 ± 10) μm and (141 ± 14) μm in the group C,(76 ± 12) μm and (141 ± 15) μm in the E group,respectively.And there was no significant difference in the thickness of corneal epithelium and full-thickness of the cornea before treatment and 2 weeks after treatment in the group A,B and D (all P ＞ 0.05),but both variables were markedly thickened in group C and E 2 weeks after treatment,and the difference was statistically significant (all P ＜0.05).Moreover,the increased levels on the both variables in the group E was higher than those in the group C 2 weeks after treatment,and the difference was statistically significant (both P ＜ 0.05).The average thickness of corneal epithelium and full-thickness of the cornea in the group C and E were significantly thickened after treatment,and the difference was statistically significant (all P ＜ 0.05).The average values of both variables in the group C and E were obviously larger than those in the group A,and the difference was statistically significant (all P ＜ 0.05).Conclusion Citalopram alone has no significant effects on the corneal thickness by OCTA,whereas both the thickness of corneal epithelium and fullthickness of the cornea tend to thicken by benzalkonium bromide treatment,which has a synergistic effect on corneal thickening with citalopram.关键词
光学相干断层扫描血管造影技术/苯扎溴铵/西酞普兰/角膜厚度Key words
optical coherence tomography angiography/benzalkonium bromide/citalopram/cornea分类
医药卫生引用本文复制引用
闵幼兰,邵毅,袁晴,马明洋,刘康成,朱佩文,姜楠,徐晓玮,申眉,叶蕾..苯扎溴铵和西酞普兰对小鼠角膜上皮和角膜全层厚度的影响[J].眼科新进展,2018,38(2):101-105,5.基金项目
国家自然科学基金资助(编号:81660158、81160118、81460092、81400372) （编号:81660158、81160118、81460092、81400372）
江西省远航工程(编号:2014022) （编号:2014022）
江西省自然科学基金重大项目(编号:20161ACB21017) （编号:20161ACB21017）
江西省青年科学基金(编号:20151BAB215016) （编号:20151BAB215016）
江西省科技支撑计划项目(编号:20151BBG70223) （编号:20151BBG70223）
江西省卫计委科技计划面上项目(编号:20175115、20175116)National Natural Science Foundation of China (No:81660158,81160118,81460092,81400372) （编号:20175115、20175116）
Jiangxi Province Voyage Project (No:2014022) （No:2014022）
Natural Science Key Project of Jiangxi Province (No:20161ACB21017) （No:20161ACB21017）
Youth Science Foundation of Jiangxi Province (No:20151BAB215016) （No:20151BAB215016）
Technology and Science Foundation of Jiangxi Province (No:20151BBG70223) （No:20151BBG70223）
Health Development Planning Commission Science Foundation of Jiangxi Province(No:20175115,20175116) （No:20175115,20175116）
