眼科新进展2018，Vol.38Issue(2)：131-135,5.DOI:10.13389/j.cnki.rao.2018.0028
PFKFB3、S1P2在2型糖尿病大鼠视网膜中的表达及tBHQ的干预作用
Expression of PFKFB3 and S1P2 in the retina of type 2 diabetic rats and the intervention effect of tBHQ
摘要
Abstract
Objective To investigate the expression of 6-phosphofructo-2-kinase /fructose-2,6-bisphosphatase-3 (PFKFB3) and sphingosine 1-phosphate receptor 2 (S1 P2) in the retina of rats with type 2 diabetes mellitus (DM) and to explore the correlation of tertiary butylhydroquinone (tBHQ) with PFKFB3 and S1P2.Methods Together 60 male SD rats were divided into normal control group (NC group),diabetes mellitus group (DM group) and tBHQ group.Type 2 DM model was induced in the latter two groups.The rats in tBHQ group were given 10 g · L-1 tBHQ in high-fat and highsugar diet 1 week after successful modeling,while DM rats were fed with high-fat and high-sugar diet continuously.At 4 weeks and 12 weeks after tBHQ intervention,blood samples were taken from the hearts of rats in each group,and serum contents of fasting plasma glucose (FPG) and fasting serum insulin (FINs) were measured.Immunohistochemistry and qRT-PCR were taken to detect the distribution and expression of PFKFB3,S1 P2 and vascular endothehal growth factor (VEGF) mRNA and protein in the retina,and TUNEL methods were used to detect apoptosis index of retinal ganglion cells of rats in each group.Results There were significant difference in the FPG and FINs levels of the three groups at 4 weeks and 12 weeks (all P =0.000).Light microscopy test showed that positive expressions of PFKFB3,S1 P2 and VEGF protein were found in all groups at 4 and 12 weeks,which were mainly located in the retinal ganglion cell layer and the inner nuclear layer.Immunohistochemistry and qRT-PCR showed that the difference in relative expressions of PFKFB3,S1P2,VEGF protein and mRNA in the retina at different times after modeling were statistically significant (all P ＜ 0.05).At 4 and 12 weeks,the expression levels of PFKFB3,S1P2 and VEGF in DM group were higher than those in NC group,but their expressions in tBHQ group were significantly downregulated when compared with DM group,and all differences were statistically significant (all P ＜ 0.05).Compared with 4 weeks,PFKFB3,S1 P2 and VEGF mRNA and protein in DM group were overexpressed at 12 weeks,and the expression level of S1 P2 in tBHQ group at 12 weeks was lower than that at 4 weeks,and the differences were statistically significant (all P ＜ 0.05).TUNEL assays showed that there was significant difference in the apoptotic index (AI) of retinal ganglion cells of rats in each group at different times after modeling (all P ＜ 0.05).DM group had higher AI than NC group (P ＜ 0.05),and tBHQ group was lower than DM group (P ＜ 0.05);Compared with 4 weeks,DM group had increased AI at 12 weeks while tBHQ group had decreased AI (both P ＜ 0.05).Conclusion PFKFB3,S1P2 and VEGF are involved in the pathological process of the retina of type 2 DM rats,which may play a role through the PFKFB3 / VEGF / S1P2 signaling pathway and may be related to the apoptosis of retinal ganglion cells.And it is indicated that tBHQ has a protective effect on the retina of type 2 DM rats.关键词
6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3/1-磷酸鞘氨醇受体2/叔丁基对苯二酚/糖尿病/视网膜Key words
PFKFB3/S1P2/tBHQ/diabetes/retina分类
医药卫生引用本文复制引用
田敏,余曦,吕红彬..PFKFB3、S1P2在2型糖尿病大鼠视网膜中的表达及tBHQ的干预作用[J].眼科新进展,2018,38(2):131-135,5.基金项目
四川省教育厅基金资助项目(编号:17ZA0428)Project of Education Department in Sichuan Province (No:17ZA0428) （编号:17ZA0428）
