中国耳鼻咽喉颅底外科杂志2018，Vol.24Issue(1)：33-38,6.DOI:10.11798/j.issn.1007-1520.201801008
EphA2经P-糖蛋白调控鼻咽癌紫杉醇敏感性的实验研究
EphA2 promotes paclitaxel resistance via P-gp in nasopharyngeal carcinoma in vitro
摘要
Abstract
Objective To investigate the role of P-glycoprotein (P-gp) in EphA2-mediated paclitaxel resistance in nasopharyngeal carcinoma (NPC) in vitro.Methods EphA2 expression in NPC cell lines was modulated by lentiviral vector,P-gp expression was detected by western blotting accordingly.P-gp was down-regulated in normal or EphA2 overexpressed NPC cell lines and its survival rate after paclitaxel treatment was determined by CCK-8.PI3K/Akt specific inhibitor LY294002 was added to EphA2 up-regulated NPC cells,the expressions of Akt,p-Akt and P-gp were determined by western blotting after 48h paclitaxel treatment.Results The expression of P-gp was down-regulated in the normal EphA2 group and up-regulated in the overexpressed EphA2 group.After paclitaxel treatment,the survival rate of NPC cells in P-gp knock down group (52.1 ±-3.7％) was significantly lower than those of the negative control group (72.4 ± 6.2％) and blank control group (68.9 ± 4.0％) (both P ＜ 0.05).Similar results were also observed in overexpressed EphA2 group [(36.1 ± 5.1％) vs (58.2 ±5.8％);(36.1 ± 5.1％) vs(57.9 ±6.2％) (both P ＜0.05)].In paclitaxel treated EphA2 overexpressed NPC cell line,LY-294002 inhibited the expressions of p-Akt and P-gp but not that of total Akt.Conclusion EphA2 modulates paclitaxel resistance in NPC cells via regulation of P-gp,and PI3K/Akt path way may be involved in this process.关键词
EphA2/P-糖蛋白/紫杉醇/耐药/鼻咽癌Key words
EphA2/P-gp/Paclitaxel/Chemoresistance/Nasopharyngeal neoplasm分类
医药卫生引用本文复制引用
卢善翃,田勇泉,邱元正,刘勇,王芸芸,李果,刘超,佘笠,魏明,张叠阔,黄东海,张欣..EphA2经P-糖蛋白调控鼻咽癌紫杉醇敏感性的实验研究[J].中国耳鼻咽喉颅底外科杂志,2018,24(1):33-38,6.基金项目
国家自然科学基金(81372426,81773243,81602684,81772903) （81372426,81773243,81602684,81772903）
湖南省自然科学基金(2015JJ3137). （2015JJ3137）
