中国防痨杂志2017，Vol.39Issue(12)：1303-1308,6.DOI:10.3969/j.issn.1000-6621.2017.12.010
高敏感度酶联免疫吸附测定法检测分泌蛋白MPB64对于活动性肺结核诊断的价值
Diagnostic value of high-sensitivity ELISA in active tuberculosis through detecting the secretion protein MPB64
摘要
Abstract
Objective The aim of this study was to evaluate the value of high-sensitivity-ELISA test (HI-ELISA) in detecting Mycobacterium tuberculosis (MTB) in sputum,which is based on detecting the secretory protein MPB64.Methods Ninety seven stored sputum specimens from suspected tuberculosis patients were subjected to cultivation in BACTEC MGIT 960 tubes,Xpert MTB/RIF test and HI-ELISA test,respectively,the results of HI ELISA test and 960-liquid-culture test were compared to evaluate the diagnostic efficacy of HI-ELISA method.Results Among the 97 sputum specimens collected in this study,the positive rates of detecting MTB of 960-1iquid-culture method and HI ELISA method were 36.08％ (35/97) and 32.99％ (32/97) respectively.When compared with liquid-culture,the sensitivity of HI-ELISA was 88.57％ (31/35),the specificity of HI-ELISA was 98.39％ (61/62) and the overall coincidence rate was 94.85％ (92/97).Of the 66 positives putum specimens using Xpert MTB/RIF,the positive rate of 960-liquid-culture method and HI-ELISA method in detecting MTB were 51.51％ (34/66) and 48.48 ％ (32/66),respectively;furthermore,compared with 960 liquid culture,the sensitivity of HI-ELISA was 91.18％ (31/34),the specificity was 96.88％ (31/32) and the coincidence rate was 93.93％ (62/66).Among the 31 negative sample stested by Xpert MTB/RIF,MTB was detected in only one sample by 960 liquid-culture method but HI-ELISA method failed to detect it,however,in the other 30 sputum samples,the results of HI-ELISA method were entirely consistent with the results of 960-liquid culture method and the coincidence rate was 96.77％ (30/31).Conclusion HI-ELISA assay was able to detecting viable MTB with high sensitivity and specificity,it was of high evaluate in diagnosis and treatment of active tuberculosis.关键词
分枝杆菌,结核/分泌蛋白,MPB64/酶联免疫吸附测定/敏感性与特异性/诊断Key words
Mycobacterium tuberculosis/Secretory protein, MPB64/Enzyme-linked immunosorbentassay/Sensitivity and specificity/Diagnosis引用本文复制引用
赵冰,侯萍,黄静,贺文从,欧喜超,刘东鑫,赵雁林..高敏感度酶联免疫吸附测定法检测分泌蛋白MPB64对于活动性肺结核诊断的价值[J].中国防痨杂志,2017,39(12):1303-1308,6.基金项目
国家科技重大专项(2014ZX10003002) （2014ZX10003002）
