中国临床心理学杂志2018，Vol.26Issue(1)：43-46,4.DOI:10.16128/j.cnki.1005-3611.2018.01.010
慢性应激抑郁模型大鼠海马Trek-1、GFAP表达情况及氟西汀的干预作用
Expression of TREK-1 and Glial Fiber Acidic Protein in Hippocampus of Rats with Depression Induced by Chronic Unpredictable Stress and the Effect of Fluoxefine
摘要
Abstract
Objective:To explore the expression of Trek-1,Glial Fiber Acidic Protein(GFAP) in hippocampus of rats with depression induced by Chronic Unpredictable Stress(CUS),and the effect of fluoxetine.Methods:12 rats were randomly selected from 48 adult SD rats as control group,others were performed as the depression model with CUS.After 21 d,the model rats were randomly divided into CUS group,fluoxetine low and high dose groups.Sucrose consumption test and openfield test were used to assess the behavior changes.The expression of Trek-1 and GFAP mRNA in hippocampus were measured with RT-PCR.Cell apoptosis was observed with TUNEL staining.Results:Before treatment,the consumption of sucrose,percentage of sucrose consumption,scores of vertical and horizontal movement were significantly lower in the CUS,fluoxetine low and high dose groups than control group.After treatment,compared with the control group,the above data in the CUS group decreased remarkably.However,the above data in the fluoxetine low and high dose groups were significantly higher than that in CUS group and before treatment,but still lower than that in the control group.Compared with the control group,the ratio of cell apoptosis and expression of Trek-1 mRNA increased significantly,while the GFAP mRNA decreased significantly in the CUS group.Compared with the CUS group,the ratio of cell apoptosis and expression of Trek-1 mRNA decreased significantly,while the GFAP mRNA increased significantly in the fluoxetine low and high dose groups.Conclusion:Fluoxetine can change the expression of Trek-1 and GFAP,inhibit the cell apoptosis,and subsequently improve the depression symptoms in rats.关键词
慢性不可预知性应激/抑郁/Trek-1/GFAP/细胞凋亡Key words
Chronic unpredictable stress/Depression/TREK-1/Glial Fiber Acidic Protein/Cell apoptosis分类
医药卫生引用本文复制引用
杨栋,喻妍,杨萍,彭红莉,傅锦华,刘丽妮..慢性应激抑郁模型大鼠海马Trek-1、GFAP表达情况及氟西汀的干预作用[J].中国临床心理学杂志,2018,26(1):43-46,4.基金项目
湖南省科技厅一般项目(2014TT2013,2013FJ3118) （2014TT2013,2013FJ3118）
湖南省科技厅重点研发计划项目(2015SK2032) （2015SK2032）
湖南省教育厅一般项目(15C0835) （15C0835）
湖南省人民医院2015年度仁术基金项目 （）
