中国临床药理学与治疗学2017，Vol.22Issue(12)：1332-1339,8.
苯那普利干预Wnt信号通路抑制UUO大鼠肾纤维化的实验研究
Experimental study of benazepril intervene Wnt signaling inhibits renal fibrosis in UUO rats
摘要
Abstract
AIM:To investigate the antagonism mechanism of benazepril on renal fibrosis of unilateral uretreal obstructire (UUO).METHODS:Fiftyfour Sprague-Dawley (SD) rats were randomly divided into three groups:sham operation group,model group,benazepril group.All the other groups were treated with surgery method of unilateral ureteral ligation to establish a rat model of renal fibrosis except the sham operation group.The pathological changes were observed by Masson staining;the positive staining expression of TGF-β1,Col-Ⅳ,Wnt1,Wnt4 in frozen sections were observed by immunofluorescence staining;the expressions TGF-β1,Col-Ⅳ,Wnt1 and Wnt4 mRNA in renal tissues were detected by real time fluorescence quantitative PCR.RESULTS:Masson staining results:compared with the sham group,the parts of blue dye collagen fiber and the degree of renal fibrosis increased significantly in all modeled groups;compared with the model group,the parts of blue dye collagen fiber and the degree of renal fibrosis decreased significantly in benazepril group.Immunofluorescence and PCR results:compared with the model group,the positive expression of Wnt1,Wnt4,TGF-β1,Col-Ⅳ and mRNA in the benazepril group decreased significantly.CONCLUSION:Benazepril can improve renal fibrosis by inhibiting the expression of TGF-β1,Col-Ⅴ,Wnt1,Wnt4 and ameliorating the level of renal fibrosis.关键词
苯那普利/肾纤维化/单侧输尿管结扎/Wnt信号通路Key words
benazepril/renal interstitial fibrosis/unilateral ureteral obstruction/Wnt signaling分类
医药卫生引用本文复制引用
赵燕云,吴小燕,汪国敏,冯雨林,董飞侠..苯那普利干预Wnt信号通路抑制UUO大鼠肾纤维化的实验研究[J].中国临床药理学与治疗学,2017,22(12):1332-1339,8.基金项目
浙江省自然科学基金(LY14H270001) （LY14H270001）
浙江省151人才专项 （）
温州市551人才专项 （）
温州市高层次人才课题资助 （）
浙江省中医药管理局课题资助(2013ZB121) （2013ZB121）
