中国临床药理学与治疗学2017，Vol.22Issue(12)：1340-1345,6.
游泳力竭对冠脉结扎致大鼠慢性心衰模型的优化
Optimization of chronic heart failure model induced by coronary artery ligation in rats with swimming exhaustion
摘要
Abstract
AIM:To establish the rat model of ischemic chronic heart failure by coronary artery ligation combining with exhaustive swimming.METHODS:40 adult rats were treated with coronary artery ligation,after 4 weeks cardiac function measurement were conducted by ultrasonography.Rats with LVEF below 40％ are considered as successful model duplication.11 rats were collected for the coronary artery ligation group,while the rest (whose LVEF were bigger than 40％) were pushed to swim for 1h per day by 15 days to promote the model formation which 8 rats were collected for exhaustion with ligation group.Left ventricular function indexes,brain natriuretic peptide (BNP) and cardiac histomorphologic changing were checked,and compared with the Control group (10 rats).RESULTS:LVEF of exhaustion with ligation group was (38.70 ± 10.10) ％,coronary artery ligation group (39.20 ± 11.10)％,which was obviously decreasing (P ＜ 0.01) compared with that of the control group (84.60 ± 3.64) ％.LVEDP of exhaustion with ligation group was (11.5 ± 1.3) mm Hg,coronary artery ligation group [(10.68 ± 4.45)mm Hg],which was obviously increasing (P ＜ 0.01)compared with that of the Control group [(4.4 ± 0.2) mm Hg].The BNP level of exhaustion with ligation group was (561.0 ± 21.0) μg/L,coronary artery ligation group (548.6 ± 25.8) μg/L,which was obviously increasing (P ＜0.01) compared with that of control group [(366.2 ± 21.8) μg/L].There are lots of red myocardial cells with stripe clear in the control group based on Masson's trichrome staining,but there are so many blue collagenous fibers instead of myocardial cells in exhaustion with ligation group and coronary artery ligation group.The standard-reaching rate of model was about 35％ at 4 weeks after operation,while final standard-reaching rate rose to about 62％ after exhausting swimming.Although the difference between the indexes of the coronary artery ligation group and the post ligation group was not significant,the rate of improvement was significant (P ＜ 0.01).CONCLUSION:Ligation of coronary artery combined with swimming exhaustion can establish ischemic chronic heart failure model,which is more economic and can obtain high success rate,thus is suitable to generalization.关键词
冠状动脉结扎/游泳力竭/心力衰竭/大鼠Key words
coronary artery ligation/exhaustive swimming/heart failure/rat分类
医药卫生引用本文复制引用
刘长江,武洋,邵培培,李少华,程路峰..游泳力竭对冠脉结扎致大鼠慢性心衰模型的优化[J].中国临床药理学与治疗学,2017,22(12):1340-1345,6.基金项目
国家自然科学基金资助项目(81360491) （81360491）
自治区高校科研计划(重点项目)(XJEDU2013I22) （重点项目）
