中国临床药理学与治疗学2017，Vol.22Issue(12)：1346-1351,6.
右美托咪定对A549细胞缺氧/复氧损伤时JNK的影响
Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury
摘要
Abstract
AIM:To investigate the effects and c-Jun N-terminal kinase(JNK) expression of dexmedetomidine (DEX) on A549 cells with hypoxia/reoxygenation (H/R) injury.METHODS:A549 cells were cultivated and were randomly divided into four groups (n =10):control group (N),DEX group (D),hypoxia/reoxygenation injury group (H),hypoxia/reoxygenation injury + DEX interfere group (HD).After all models were completed,the morphological changes of A549 cells were observed under the inverted microscope.Cell activity was detected by CCK-8 and the apoptosis index (AI) was detected by in situ end labeling (TUNEL) method.The expression of GRP78,p-JNK,caspase-3 at protein levels and GRP78,JNK mRNA were detected by Western blot and RT-PCR.RESULTS:Compared with N group,the number of adherent cells in H group decreased significantly,and cell morphology changed.The expression of OD value in H group decreased obviously (P ＜ 0.01),the expression of AI value,GRP78,p-JNK,caspase-3 protein and GRP78,JNK mRNA were significantly increased (P＜ 0.01).HD group compared with H group,the cell damage alleviated,the expression of OD value was increased (P ＜ 0.01),the number of apoptosis cells and the AI value in HD group were significantly decreased (P ＜ 0.01).Dramatically decreased the expression of p-JNK,caspase-3 protein and JNK mRNA (P ＜ 0.01).CONCLUSION:DEX can effectively alleviate A549 cells damage induced by H/R injury,which may be related to inhibition of the JNK pathway.关键词
JNK/右美托咪定/缺氧/复氧损伤/A549细胞/细胞凋亡Key words
JNK/dexmedetomidine/hypoxia/reoxygenation injury/A549 cells/cell apoptosis分类
医药卫生引用本文复制引用
罗梓垠,项冰倩,高慧,邱晓晓,钱小英,王万铁..右美托咪定对A549细胞缺氧/复氧损伤时JNK的影响[J].中国临床药理学与治疗学,2017,22(12):1346-1351,6.基金项目
浙江省公益技术应用研究项目(2013C33168) （2013C33168）
浙江省新苗人才计划项目(2014R413043) （2014R413043）
温州市公益性科技计划项目(Y20140652) （Y20140652）
