中国临床药理学与治疗学2017，Vol.22Issue(12)：1364-1370,7.
雷公藤多苷增强耐顺铂人上皮性卵巢癌SKOV3/DDP细胞株顺铂敏感性的体外研究
Study on the cisplatin-sensitization effect of tripterygium glycosides on cisplatin-resistant human epithelial ovarian cancer cells (SKOV3/DDP) in vitro
摘要
Abstract
AIM:To study the cisplatin-sensitization effect and mechanism of tripterygium glycosides on cisplatin-resistant human epithelial ovarian cancer cells (SKOV3/DDP).METHODS:The SKOV3/DDP cells in exponential phase of growth were randomly divided into eight groups:blank control group,10 μg/mL DDP group,50 μg/mL GTW group,800 μg/mL GTW group,3 200 μg/mL GTW group,10 μg/mL DDP + 50 μg/mL GTW group,10 μg/mL DDP + 800 μg/mL GTW group and 10 μg/mL DDP + 3 200 μg/mL GTW group.Cell counting kit 8 and flow cytometry and western blot were used to detect the growth inhibiting rate and apoptosis rate and relative expression of GST-π,MDR1,STAT3,p-STAT3 of SKOV3/DDP cells of every group.RESULTS:DDP and GTW produce an additive effect when used concurrently in inhibiting growth of SKOV3/DDP cells.800 μg/mL GTW or 3 200 μg/mL GTW combined with 10 μg/mL DDP can significantly induce apoptosis of SKOV3/DDP cells and downregulate the expression of p-STAT3 (P ＜ 0.05).CONCLUSION:GTW can significantly enhance sensitivity of SKOV3/DDP cells to DDP.The underlying mechanism may be related with down-regulating the expression of p-STAT3 in STAT3 pathway.关键词
雷公藤多苷/SKOV3/DDP/顺铂/增敏作用/STAT3Key words
tripterygium glycosides/SKOV3/DDP/cisplatin/cisplatin-sensitization effect/STAT3分类
医药卫生引用本文复制引用
刘文成,谭布珍,占欣璐,胡辉,黄根华,曹青..雷公藤多苷增强耐顺铂人上皮性卵巢癌SKOV3/DDP细胞株顺铂敏感性的体外研究[J].中国临床药理学与治疗学,2017,22(12):1364-1370,7.基金项目
江西省自然科学基金(20161BBG70218) （20161BBG70218）
江西省卫生计生委中医药管理局科研基金(2016A078) （2016A078）
