中国临床药理学与治疗学2017，Vol.22Issue(12)：1377-1381,5.
RGS4和D2受体信号通路相关分子在甲基苯丙胺依赖CPP大鼠纹状体的表达变化
Expression of RGS4 and D2 receptor signaling pathway in striatum of methamphetamine-dependent CPP rats
摘要
Abstract
AIM:To study the expression of signal transduction molecules in the striatum G protein protein 4 (RGS4) and dopamine D2 receptor (D2) in conditioned place preference (CPP) rats treated with methamphetamine (meth).METHODS:METH dependence CPP model was established (1 week and 2 weeks of METH dependence groups),The protein expression of RGS4 and D2,inhibitory G protein alpha-subunit (Gαi),mitogenactivated protein kinase (MAPK) in striatum were determined by Western blotting (WB).The changes of cyclic adenosine monophosphate (cAMP) content in striatum of rats were determined by enzyme linked immunosorbent assay (ELISA).RESULTS:Compared with saline control group,the average time of rats in the methamphetamine-paired chamber for two groups was increased (P ＜ 0.05).Compared with saline control group,RGS4 protein expressions in the two METH dependent groups were reduced (P ＜0.01);compared with 1 week of METH dependence group,that of 2 weeks group was reduced significantly(P ＜ 0.05).D2,Gαi,MAPK protein and cAMP expressions in the two METH dependent groups were increased (P ＜ 0.01);compared with 1 week of METH dependence group,those of 2 weeks were increased significantly (P ＜ 0.05).CONCLUSION:RGS4 and D2 receptor signaling pathways in striatum have changed in METH dependent rats,RGS4 may be involved in the regulation of METH-dependent D2 receptor signaling pathway in METH dependent rats.关键词
甲基苯丙胺/条件性位置偏爱/G蛋白信号调节因子4/多巴胺D2受体/抑制性G蛋白α亚基/丝裂原激活的蛋白激酶/环磷酸腺苷Key words
methamphetamine/CPP/RGS4/D2/Gαi/MAPK/cAMP分类
医药卫生引用本文复制引用
蒙国懿,何繁漪,隋念含,赵林波,洪仕君,邢豫明,赵永娜..RGS4和D2受体信号通路相关分子在甲基苯丙胺依赖CPP大鼠纹状体的表达变化[J].中国临床药理学与治疗学,2017,22(12):1377-1381,5.基金项目
云南省应用基础研究计划联合专项基金资助项目(2012FB023) （2012FB023）
