中国临床药理学与治疗学2018，Vol.23Issue(1)：8-12,28,6.DOI:10.12092/j.issn.1009-2501.2018.01.002
柠檬苦素对脂多糖致小鼠急性肺损伤的作用
Effects of limoninon on LPS-induced acute lung injury in mice
摘要
Abstract
AIM:To investigate the effects of limonin (LM) on lipopolysaccharides (LPS)-induced acute lung injury (ALI) in mice.METHODS:Forty ICR male mice were divided randomly by weight into 4 groups:normal group,model group,dexamethasone (Dex)-treated group and LM-treated group.Each group had ten mice.Dex and LM were intraperitoneally injected at the dose of 10 mg/kg,respectively.Two hours after drug administration,except the mice in normal group treated with same vehicle,others were intracheally injected with LPS (5 mg/kg).Six hours after LPS challenge,mice were sacrificed and lung tissues were obtained for calculating the pulmonary index and wet/dry mass (W/D) ratio.The sections were stained with hematoxylin and eosin (HE) for histological analysis.The levels of TNF-αt,IL-1β,and IL-6 in serum were determined by ELISA.Myeloperoxidase (MPO) in serum were determined by chromatometry.The mRNA expressions of TNF-α,IL-1β and IL-6 were determined by RT-PCR while TLR4 and NF-κB p65 protein by Westem blot analysis.RESULTS:Compared with normal group,mice in model group had obvious increase of lung indexes and W/D ratios,which were accompanied by marked inflammatory cell infiltration,alveolar congestion and edema in lung;the levels of TNF-α,IL-1 β,IL-6 and MPO in serum as well as their mRNA expressions in the lung of ALI mice were also increased (P ＜ 0.01).Compared with model group,lung indexes and W/D ratios were decreased in LM-treated group while histological changes were improved;the levels of TNF-α,IL-1 β,and IL-6 in serum (P ＜ 0.01) and their mRNA expressions,and the expressions of TLR4 and NF-κB p65 protein in lung tissues were also significantly decreased (P＜ 0.01).CONCLUSION:LM protects against LPS-induced ALI via regulating TLR4/NF-κB pathway and inhibiting expression of inflammatory cytokines in mice.关键词
柠檬苦素/抗炎作用/脂多糖/急性肺损伤Key words
limonin/anti-inflammatory effect/lipopolysaccharides/acute lung injury分类
医药卫生引用本文复制引用
王棣,张欢欢,方杰,钟宇森,余陈欢..柠檬苦素对脂多糖致小鼠急性肺损伤的作用[J].中国临床药理学与治疗学,2018,23(1):8-12,28,6.基金项目
国家自然科学基金项目(81473339,81673583) （81473339,81673583）
浙江省科技厅院所专项(2014F10033) （2014F10033）
