中国临床药理学与治疗学2018，Vol.23Issue(1)：13-17,5.DOI:10.12092/j.issn.1009-2501.2018.01.003
氧化苦参碱通过调控上皮间质转化抑制三阴性乳癌细胞侵袭转移
Oxymatrine inhibits invasion and metastasis of triple-negative breast cancer cells by regulating epithelial mesenchymal transition
摘要
Abstract
AIM:To study the inhibitory effects of oxymatrine on growth and migration of human triple-negative breast cancer MDA-MB-231 cell.METHODS:CCK-8 assay was used to detect the inhibitory effects of oxymatrine on the growth of MDA-MB-231 cells.Inhibitory effect of oxymatfine on cell cycle of MDA-MB-231 cells was detected by flow cytometry (FCM).Effect of oxymatrine on migration of MDA-MB-231 cells was detected by scratch experimental.RESULTS:Compared with the control group,the growth of MDA-MB-231 cells decreased significantly in a time-and dose-dependent manner after oxymatrine treatment (2,4,6,8,10,20 mg/mL for 24,48 and 72 h).Oxymatrine significantly increased the number of cells in G0/G1 phase and decreased the number of cells in S and G2/M phase (P ＜ 0.05).Transwell showed that oxymatrine treated MDA-MB-231 cells for 12 h significantly inhibited the invasion of MDA-MB-231.The high concentration of oxymatrine significantly up-regulated the expression of E-cadherin and inhibited the expression of Snail-2 mRNA (P ＜ 0.01).CONCLUSION:Oxymatrine exerts its antitumor effect by inhibiting the growth and invasion of MDA-MB-231 cells,which plays a potential role on antitumor growth and metastasis.关键词
氧化苦参碱/人三阴性乳腺癌/上皮间质转化/侵袭/生长抑制Key words
oxymatrine/triple-negative breast cancer cells/EMT/migration/growth inhibition分类
医药卫生引用本文复制引用
蔡加琴,魏晓霞,黄旭慧,庄捷,孙红..氧化苦参碱通过调控上皮间质转化抑制三阴性乳癌细胞侵袭转移[J].中国临床药理学与治疗学,2018,23(1):13-17,5.基金项目
国家自然科学基金(81403021) （81403021）
福建省卫生计生青年科研课题(2016-1-7) （2016-1-7）
福建医科大学启航基金项目计划(2016QH107) （2016QH107）
