中国临床药理学与治疗学2018，Vol.23Issue(1)：18-23,6.DOI:10.12092/j.issn.1009-2501.2018.01.004
双PI3K/mTOR抑制剂NVPBEZ235对宫颈癌Hela细胞的放射增敏作用
Radio sensitizing effect of double PI3K/mTOR inhibitor NVPBEZ235 on Hela cells of cervical cancer
摘要
Abstract
AIM:To investigate the effect that external irradiation of dual PI3K/mTOR inhibitors NVPBEZ235 on cervical cancer Hela cells.METHODS:Cervical cancer Hela cells treated with different concentrations of NVPBEZ235 and the radiotherapy (irradiation,IR) were examined for cell viability using MTT assay and colony forming ability.The cycle arrest of cervical cancer cells combined drug with IR was analyzed by using the flow cytometry.RESULTS:Compared with the control group,NVPBEZ235 can inhibit human cervical carcinoma cell in vitro (P ＜ 0.05).The combined use of NVPBEZ235 significantly enhanced the inhibitory effect of IR (P＜0.05) in Hela cells.In vitro,NVPBEZ235 and IR,which had synergistic effect,can block cell cycle to G2/M period by the combined effect which was significantly greater than the effect of one single drug (P ＜ 0.05).CONCLUSION:20％ IC50 NVPBEZ235 can promote the cell's radiation sensitivity,inhibit the proliferation of cells,and thereby,increase its radio sensitivity to cervical cancer cells.关键词
双PI3 K/mTOR抑制剂/NVPBEZ235/Hela/放射增敏Key words
dual PI3K/mTOR inhibitor/NVPBEZ235/Hela/radio sensitization分类
医药卫生引用本文复制引用
郑迎奥,李胜泽,张雷,郭祥瑞,鲍佳茜,吴涛,刘健..双PI3K/mTOR抑制剂NVPBEZ235对宫颈癌Hela细胞的放射增敏作用[J].中国临床药理学与治疗学,2018,23(1):18-23,6.基金项目
安徽省教育厅自然科学基金(KJ2015B041,KJ2015B096) （KJ2015B041,KJ2015B096）
大学生创业创新项目(19262,201610367002) （19262,201610367002）
