中国临床药理学与治疗学2018，Vol.23Issue(1)：34-40,7.DOI:10.12092/j.issn.1009-2501.2018.01.007
匹诺塞林对脑缺血再灌注内质网应激凋亡相关基因mRNA表达的影响
Effects of pinocembrin on mRNA expression of genes related to endoplasmic reticulum stress-mediated apoptosis in acute injury induced by focal cerebral ischemia-reperfusion
摘要
Abstract
AIM:To investigate the effects of pinocembrin on mRNA expression of several genes related to endoplasmic reticulum stress-mediated apoptosis in acute injury induced by focal cerebral ischemia-reperfusion.METHODS:Fifty male Sprague-Dawley rats,weighing 260-280 g were divided into five groups:sham operation group;I/R group;I/R + pinocembrin (1,3 and 10 mg/kg) group.Focal cerebral I/R model was established by occlusion of the middle cerebral artery (MCAO) for 2 h followed by 6 h reperfusion.Pinocembrin was administered intravenously at different doses at the onset of reperfusion.The mRNA expression of gene GRP78,CHOP,ATF4,XBP-1 was then detected by RT-PCR method in penumbra cortex,respectively.RESULTS:Pinocembrin significantly modulated the levels of mRNA expression of these genes by increasing GRP78 and attenuating CHOP expression along with ATF4,and XBP-1 (P ＜ 0.05 or P ＜ 0.01).CONCLUSION:Pinocembrin can protect against L/R injury,and the possible mechanism might be attributed to its regulation of mRNA expression of genes related to endoplasmic reticulum stress-mediated apoptosis in penumbra area.关键词
匹诺塞林/内质网应激/脑缺血再灌注/GRP78/CHOP/ATF4/XBP-1Key words
pinocembrin/endoplasmic reticulum stress/focal cerebral ischemia reperfusion/GRP78/CHOP/ATF4/XBP-1分类
医药卫生引用本文复制引用
武彩霞,杜冠华..匹诺塞林对脑缺血再灌注内质网应激凋亡相关基因mRNA表达的影响[J].中国临床药理学与治疗学,2018,23(1):34-40,7.基金项目
山东省医药卫生科技发展计划项目(2016WS0566) （2016WS0566）
