中国临床药理学杂志2017，Vol.33Issue(23)：2427-2430,4.DOI:10.13699/j.cnki.1001-6821.2017.23.029
Folin-Ciocalteu法测定金线莲叶中总多酚的含量
Determine the content of total polyphenols in Anoectochilus roxburghii leaves by Folin-Ciocalteu colorimetry
摘要
Abstract
Objective To establish a Folin-Ciocalteu (FC) colorimetric method to determine the total polyphenols content of Anoectochilus roxburghii leaves.Methods The concentration of FC reagent and Na2CO3 reagent,reacting temperature and duration were evaluated respectively with gallic acid as reference and detecting wavelength 751 nm.The stability,precision and recovery rate of the method were also tested.Results The optimal conditions of FC colorimetry were:sample solution 1 mL,30％ FC reagent 5 mL and 10％ Na2CO3 10 mL,reacting temperature 25 ℃,reaction time 100 min.The calibration curve of polyphenols was well linear in the range of 0-50 μg · mL-1 (r =0.999 8) and the stability,precision and average recovery rate were 1.45％,1.18％ and 3.79％ by RSD (relative standard deviation).The average recovery rate was 101.10％.Conclusion The stability,precision and recovery of FC colorimetry were established well.The method is convenient and accurate,and it is reliable to be applied to determine total polyphenols content in Anoectochilus roxburghii leaf extracts.关键词
金线莲叶/多酚/Folin-Ciocalteu比色法Key words
Anoectochilus roxburghii leaf/polyphenol/Folin-Ciocalteu colorimetry分类
医药卫生引用本文复制引用
马明,常静,林莉莉,程沁园..Folin-Ciocalteu法测定金线莲叶中总多酚的含量[J].中国临床药理学杂志,2017,33(23):2427-2430,4.基金项目
江苏省卫生职业院校教科研课题基金资助项目(JZ201510) （JZ201510）
