中国临床药理学杂志2017，Vol.33Issue(24)：2580-2583,4.DOI:10.13699/j.cnki.1001-6821.2017.24.007
参麦注射液减少大鼠肢体缺血再灌注肺损伤的作用
Function of Shenmai injection on lung injury induced by limb ischemia/reperfusion in rats
摘要
Abstract
Objective To observe the effects of Shenmai injection on plasma inflammatory cytokines and lung injury in limbs ischemia-reperfusion lung injury (LIRLI)of rats.Methods SD rats with LIRLI were randomly divided into five groups (n =10):Sham operation group,model group,experimental-L,experimental-M,experimental-H groups (1,2,3 mL · kg-1 Shenmai injection).Blood gas analysis was performed at the end of the experiment.The enzyme linked immunosorbent assay was used to measure the concentration of inflammatory cytokines in the plasma.The lung injury score was evaluated with hematoxylin eosin(HE) by microscope.Results pH value in sham operation group,model group,experinental-L,experimental-M,experimental-H groups were 7.35 ± 0.09,7.15 ± 0.08,7.25 ± 0.11,7.31 ± 0.10,7.36 ±0.14.pH value in model group was lower than sham operation group with significantly while experimental-H group was higher than model group significantly (all P ＜ 0.05).Compared sham operation group,PaO2 in model group were significantly lower and PaCO2 was significantly higher(all P ＜ 0.05).Compared with model group,PaO2 in three doses experimental groups were significantly higher and PaCO22 in three doses experimental groups was significantly lower(all P ＜ 0.05).Tumor necrosis factor-α (TNF-α) in sham operation group,model group,experimental-L,experimental-M,experimental-H groups were (1.37 ± 0.15),(6.78 ± 1.23),(5.28 ± 0.79),(3.35 ± 0.32),(2.31 ±0.25) nmol · mL-1.Compared with sham operation group,TNF-α in model group were significantly higher (P ＜ 0.05).Compared with model group,TNF-α in three doses experimental groups were significantly lower (all P ＜0.05).Interleukin(IL)-6 and IL-10 in sham operation group,model group,experimental-L,experimentalM,experimental-H groups were (160 ±24),(342 ±34),(324 ±29),(289 ±33),(253 ±37)pg · mL-1;(175±12),(120±14),(132 ±6),(145 ± 11),(155 ±15) pg · mL-1.Compared with sham operation group,IL-10 in model group were significantly lower and IL-6 was significantly higher(all P ＜ 0.05).Compared with model group,IL-6 in three doses experimental groups were significantly lower and IL-10 was significantly higher(all P ＜0.05).The lung injury score(point) in sham operation group,model group,experimental-M,experimental-H groups were 0.75 ±0.23,7.26 ± 0.52,3.65 ± 0.32,1.39 ± 0.25.The difference between sham operation group and model group while model group and experimental-M,-H groups were significantly (all P ＜ 0.05).Conclusion Pretreatment with Sheumai injection can reduce the lung injury induced through reducing the release of inflammatory cytokines in the lung.关键词
缺血再灌注/参麦注射液/炎症因子/肢体缺血再灌注肺损伤Key words
ischemia/reperfusion/Shenmai injection/inflammatory cytokines/limb ischemia-reperfusion lung injury分类
医药卫生引用本文复制引用
熊响清,王良荣,林丽娜..参麦注射液减少大鼠肢体缺血再灌注肺损伤的作用[J].中国临床药理学杂志,2017,33(24):2580-2583,4.基金项目
浙江省自然科学基金资助项目(LY16H070004) （LY16H070004）
浙江省医药卫生科技基金资助项目 (2013KYA131) （2013KYA131）
