中国临床药理学杂志2017，Vol.33Issue(24)：2588-2591,4.DOI:10.13699/j.cnki.1001-6821.2017.24.009
氯沙坦在脑内对脱钠大鼠高盐摄入的抑制作用
Inhibitory effect of losartan in the brain on hypertonic sodium intake by sodium-depleted rats
摘要
Abstract
Objective To investigate the effect of losartan on sodium appetite and its underlying mechanisms.Methods Sprague-Dawley rats,with stainless steel cannulas implanted bilaterally into the central nucleus of amygdala (CeA),were treated with water deprivation for 24 h followed voluntary drinking-water for 2 h or treated with furosemide combined with captopril injected subcutaneously in order to induce to be Na + ingestion models.The Na + ingestion models of rat were received bilateral injections of combinations of saline,[D-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin (DAMGO) 0.25 μL · nmol-1 and losartan 5 nL · nmol-1 into the CeA,and the rats were randomly assigned to four groups:saline + saline (blank group),saline + DAMGO (experimental group),losartan + saline (control group),or losartan + DAMGO (combination group).Following the CeA injections,non-cumulative 0.3 mol · L-1 NaCl intake and water intake were automatically recorded by feeding-drinking-activity analyser at 15,30,45,60,90,120,150,180,210 and 240 min (two-bottle test).Results In rats treated with water deprivation followed partial rehydration,over the entire 240 min sodium appetite tests,the cumulative total 0.3 mol · L-1 NaCl intake in blank group,experimental group,control group,and combination group were (2.01 ± 0.32),(8.04 ± 1.67),(1.75 ± 0.31),(4.26 ± 0.60) mL,respectively;the cumulative total water intake were (2.66 ± 0.66),(10.40 ± 2.69),(2.15 ±0.46),(5.34 ± 0.91) mL,respectively.Compared with the blank group,both the cumulative total NaCl intake and water intake in the experimental group increased significantly (both P ＜ 0.05).Compared with the experimental group,both the cumulative total NaC1 intake and water intake in the combination group decreased significantly (both P ＜ 0.05).In rats treated with subcutaneous injections of furosemide combined with captopril,over the entire 240 min sodium appetite tests,the cumulative total 0.3 mol · L-1 NaC1 intake in blank group,experimental group,control group,and combination group were (4.36 ±0.16),(9.42 ±2.02),(2.81 ±0.76),(5.48 ± 1.56) mL,respectively;the cumulative total water intake were (8.38 ± 0.73),(12.20 ± 1.28),(7.05 ± 0.52),(9.42 ± 1.24) mL,respectively.Compared with the blank group,both the cumulative total NaC1 intake and water intake in the experimental group increased significantly (both P ＜ 0.05).Compared with the experimental group,both the cumulative total NaC1 intake and water intake in the combination group decreased significantly (both P ＜ 0.05).Conclusion Losartan in the brain may decrease the expression of sodium appetite mediated by μ-opioid receptor system within the CeA,and then inhibits high sodium consumption by sodium-depleted rats.关键词
氯沙坦/μ-阿片受体激动药/杏仁中央核/钠欲/钠盐摄入Key words
losartan/μ-opioid receptor agonist/central nucleus of amygdala/sodium appetite/sodium intake分类
医药卫生引用本文复制引用
闫君宝,卢敏江,胡志红,任爱红,范玲玲,邓博,杨东伟..氯沙坦在脑内对脱钠大鼠高盐摄入的抑制作用[J].中国临床药理学杂志,2017,33(24):2588-2591,4.基金项目
河南省高等学校重点科研基金资助项目(15A180014) （15A180014）
