中国临床药理学杂志2017，Vol.33Issue(24)：2592-2594,2602,4.DOI:10.13699/j.cnki.1001-6821.2017.24.010
塞来昔布对颅脑损伤后生成素表达及神经元凋亡的影响
Effects of celecoxib on the expression of Survivin and neuron apoptosis after traumatic brain injury in rats
摘要
Abstract
Objectve To investigate the effect of celecoxib on the expression of Survivin and neuronal apoptosis after traumatic brain injury in rats,and to explore its brain protection mechanism.Methods The 64 SD rats were randomly divided into four groups:normal group,sham operation group,model group and experimental group.The rats in experimental group and model group were established with closed craniocerebral injury model by free falling method.In the experimental group,intraperitoneal injection of celecoxib (250 mg · kg-1) was performed immediately after the craniocerebral injury model was established.The rats in sham operation group and the normal group were given the same amount of 0.9％ NaC1 intraperitoneally.The expressions of Survivin and Caspase-3 were detected by immunohistochemistry.The neuronal apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling(TUNEL) method.Results The expression of Survivin protein in the normal group,sham-operated group,model group,and experimental group were 3.19 ± 1.43,5.34 ± 1.26,14.17 ±0.54,27.76 ±0.70.The expression of Casepse-3 protein in above four groups were 1.19 ±0.51,3.19 ±0.71,11.17 ±3.74,6.30 ±2.80.The expression of Survivin in the model group was significantly higher than in normal group and sham-operated group with significantly (all P ＜ 0.05).Compared with the model group,the expression of Survivin and Caspase-3 in the experimental group increased with significantly (all P ＜0.05).The number of apoptotic cells in 6,12,24,72 h in the sham-operated group,model group,and experimental group were 2.84 ± 0.56,12.17 ± 1.74,5.36 ± 0.80;3.19 ± 0.71,33.17 ± 3.74,26.30 ± 2.80;5.34 ± 0.83,72.57 ± 6.74,58.48 ± 4.50;2.44 ± 0.83,61.28 ± 5.36,42.56 ± 4.63.The number of apoptotic cells in the model group was significantly higher than in sham-operated group with significantly (all P ＜ 0.05).Compared with the model group,the number of apoptotic cells in the experimental group decreased with significantly (all P ＜ 0.05).Conclusion Celecoxib may inhibit Caspase-3 activation-mediated apoptosis by increasing the expression of anti-apoptotic Survivin protein.In order to reduce the neuronal apoptosis after traumatic brain injury,and play a protective role in the brain.关键词
塞来昔布/生存素/半胱氨酸天冬氨酸蛋白酶-3/颅脑损伤/神经元凋亡Key words
celecoxib/Survivin/Caspase-3/traumatic brain injury/neuron apoptosis分类
医药卫生引用本文复制引用
张金玲,张涛,国建飞,郑丽,杨群福,崔福生,张宇新..塞来昔布对颅脑损伤后生成素表达及神经元凋亡的影响[J].中国临床药理学杂志,2017,33(24):2592-2594,2602,4.基金项目
河北省自然科学基金资助项目(C2004000689) （C2004000689）
河北省博士基金资助项目(05547008D-4) （05547008D-4）
河北省科学技术与社会发展计划基金资助项目(04276135) （04276135）
