中国临床药理学杂志2017，Vol.33Issue(24)：2595-2598,4.DOI:10.13699/j.cnki.1001-6821.2017.24.011
菲牛蛭素对载脂蛋白E基因敲除小鼠动脉粥样硬化斑块的干预作用
Interventional effects of Bufrudin on atherosclerosis plaque in ApoE gene knockout mice
摘要
Abstract
Objective To study the interventional effect of Bufrudin on aorta atherosclerosis and angiogenesis in apolipoprotein E gene knockout (ApoE-/-) mice.Methods Male ApoE-/-mice on high fat diet,were randomly divided into three groups:model group,control group,experimental group.Experimental group were given Bufrudin (400 U · kg-1 · d-1),control group were given simvastatin (3 mg · kg-1 · d-1).And C57BL/6J mice fed with common diet was served as normal group.After ten weeks,mice blood was collected and serum levels of triglyceride (TG),total cholesterol(TC),low-density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) concentrations were measured.The expression of CD34 protein,vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR-2) were detected by immunohistochemstry.Results After administraiton,the serum content of TG in model group,control group and experimental group were (1.57 ± 0.12),(1.10 ± 0.21),(0.95 ±0.20) mmol · L-1,respectively;TC in above three groups were (19.93 ± 2.24),(14.47 ± t.30),(11.90 ± 2.38) mmol · L-1,respectively;LDL-C in above three groups were (17.24 ± 1.52),(13.94 ± 1.46),(10.02 ±2.14) mmol · L-1,respectively;HDL-C in above three groups were (4.05 ± 1.81),(4.62 ± 0.50),(3.38 ± 0.24) mmol · L-1,respectively.Compared with model group,the serum content of TG,TC,LDL-C,HDL-C in experimental group were significantly decreased (all P ＜ 0.01).The content of collagen in model group,control group and experimental group were (21.48 ± 2.44)％,(37.12 ± 3.33)％,(51.60 ± 2.47)％;the expression of CD34 protein in above three groups were (11.02 ± 1.64) ％,(6.82 ± 0.66) ％,(4.56 ± 0.59) ％;the expression of VEGf prtein in above three groups were (22.13 ± 2.04) ％,(16.20 ± 0.65) ％,(1 1.16 ± 0.82) ％;and the expression of VEGfR-2 in above three groups were (17.90 ± 0.73)％,(15.11 ± 0.52) ％,(10.56 ± 0.60) ％,respectively.Compared with model group,the content of collagen in experimental group was increased,and the expression of CD34 protein in experimental group was decreased,all about with statistical difference (all P ＜ 0.05).Conclusion Bufrudin attenuate atherosclerosis disease and stabilize the atherosclerotic plaque in ApoE-/-mice likely through suppressing the expression of VEGF and VEGFR-2.关键词
菲牛蛭素/动脉粥样硬化/血管新生/血管内皮生长因子Key words
Bufrudin/atherosclerosis/angiogenesis/ascular endothelial growth factor分类
医药卫生引用本文复制引用
赖吴芳,周维官,黄栎莹,张晓慧,黎渊弘..菲牛蛭素对载脂蛋白E基因敲除小鼠动脉粥样硬化斑块的干预作用[J].中国临床药理学杂志,2017,33(24):2595-2598,4.基金项目
广西科学研究与技术开发基金资助项目(桂科攻0424008-2F) （桂科攻0424008-2F）
