中国全科医学2018，Vol.21Issue(6)：670-676,7.DOI:10.3969/j.issn.1007-9572.2018.00.012
大鼠肝损伤过程中肝组织神经营养因子神经突蛋白水平变化及其意义研究
Changes in the Expression Level of Neuritin during Liver Injury in Rats and Its Significance
摘要
Abstract
Objective To investigate the changes in the expression level of neuritin (a neurotrophic factor) expressed in the liver during liver injury in rats and its significance. Methods From November 2015 to November 2016, a total of 126 healthy Sprague-Dawley rats were randomly divided into 3 groups, blank group, non-liver injury group and liver injury group with 42 in each. The liver injury model was established by a self-made modified BIM-IV biological impact machine in liver injury group, while rats of the non-liver injury group were hit with a uniform strength at the same part to cause a femoral fracture. Rats of the blank group did not receive any interventions. Seven rats were randomly selected from each group at 6 h, 12 h, 1 d, 2 d, 3 d and 5 d after the establishment of liver injury model to collect the liver specimens and serum samples. HE staining was used to detect the pathological changes in the liver tissue. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Expression levels of Neuritin expressed in the liver tissue were measured with immunohistochemical staining.Results In the liver injury group, irregular arrangement of hepatocytes, loose and balloon-shaped cytoplasms were observed at 6 h after modeling; 12 h, 1 d, and 2 d after modeling, large areas of hepatocellular necrosis and even nuclear lysis were observed;while 3, 5 days after the modeling, it was found that the arrangement of hepatocytes, morphology and size of cytoplasm and nucleus were nearly became normal. At 6 h, 12 h, 1 d, 2 d after injury, liver injury group demonstrated higher serum ALT and AST levels compared with other groups (P<0.05). In the liver injury group, the levels of ALT and AST at 12 h after modeling were significantly higher compared with 6 h after modeling (P<0.05), but both of them were higher than the following measurement levels (at 2 d, 3 d and 5 d after modeling) (P<0.05), moreover, the third measurement levels were higher than the following three measurement levels (P<0.05), and the fourth measurement levels were higher than the latter two measurement levels (P<0.05). At 6 h, 12 h, 1 d, 2 d, 3 d after modeling, liver injury group demonstrated higher Neuritin expression levels compared with other groups (P<0.05). In the liver injury group, compared with at 6 h after modeling, Neuritin expression levels were higher at 12 h, 1 d after modeling, but lower at 3 d and 5 d after modeling (P<0.05); Neuritin expression level at 12 h after modeling was higher than that measured at each of the following four times (at 1 d, 2 d, 3 d and 5 d after modeling) (P<0.05), and it was higher at 1 d after modeling than that measured at each of the following three times (at 2 d, 3 d and 5 d after modeling) (P<0.05), likewise, it was higher at 2 d after modeling than that measured at each of the following two times (at 3 d and 5 d after modeling) (P<0.05), and higher at 3 d after modeling than that of the final measurement (P<0.05). Overall ,the expression level of Neuritin in liver injury group was positively correlated with serum ALT(r=0.796 2, P<0.001) and AST (r=0.769 1, P<0.001). Conclusion After liver injury, the expression level of Neuritin in rats first increased then decreased, which changed with the same tendency as ALT and AST in serum. So, Neuritin may play a role in the regeneration or proliferation of hepatic cells after liver injury.关键词
肝疾病/神经生长因子类/丙氨酸氨基转移酶/天冬氨酸氨基转移酶类/神经突蛋白Key words
Liver diseases/Nerve growth factors/Alanine aminotransferase/Aspartate aminotransferases/Neuritin分类
医药卫生引用本文复制引用
周涛,朱美意,周杰,曹东,欧阳军..大鼠肝损伤过程中肝组织神经营养因子神经突蛋白水平变化及其意义研究[J].中国全科医学,2018,21(6):670-676,7.基金项目
国家自然科学基金资助项目(81560312) （81560312）
