中国全科医学2018，Vol.21Issue(9)：1109-1113,5.DOI:10.3969/j.issn.1007-9572.2018.00.054
"以俞调枢"法对Cajal间质细胞缺失模型大鼠胃肠动力学主要指标的影响
Effect of Yishutiaoshu Therapy on the Major Gastrointestinal Motility Parameters in a Rat Model of ICC Loss
摘要
Abstract
Objective To investigate the effect and possible mechanism of Yishutiaoshu therapy (improving the gastrointestinal motility via pressing the shu acupoints by fingers) on the major gastrointestinal motility parameters in a rat model of interstitial cells of Cajal (ICC) loss. Methods We conducted this study from January to December 2016. 120 SD cleanliness grade rats were randomly divided into the model group and blank group with 60 in each , then the 60 model rats were randomly and equally divided into the model control subgroup and model treatment subgroup, and 60 blank rats were randomly and equally divided into the blank control subgroup and blank treatment subgroup. The rats in the model group were injected monoclonal anti-c-kit antibody (ACK2) every other day for preparing the rat model of ICC loss and those in the blank group were injected the same amount of 0.9% sodium chloride solution every other day, all of them were injected 5 times. Then, both the blank treatment subgroup and model treatment subgroup were fixed and treated with yishutiaoshu therapy for 22 min once daily, but other two subgroups were only fixed for 22 min once daily, and all of them were intervened for 14 days. We observed and compared the incidence of gastric residue, small intestinal motility rate, levels of motilin, gastrin, vasoactive intestinal peptide and somatostatin between the 4 subgroups. Results Compared with both the blank control subgroup and model treatment subgroup, model control subgroup demonstrated higher incidence of gastric residues and higher mean vasoactive peptide and plasma somatostatin levels (P<0.01), but lower small intestinal motility rate, lower mean motilin and gastrin levels (P<0.01). There were no significant differences in the incidence of gastric residue, small intestinal motility rate, levels of motilin, gastrin,vasoactive intestinal peptide and somatostatin between the blank control subgroup, blank treatment subgroup and model treatment subgroup (P>0.05). Conclusion Yishutiaoshu therapy can significantly improve the gastrointestinal motility in the rat model of ICC loss, indicating that the therapy may play a role in regulating the gastrointestinal hormones(motilin, gastrin, vasoactive peptide and somatostatin).关键词
胃肠活动/间质细胞/"以俞调枢"法/胃肠激素类/大鼠Key words
Gastrointestinal motility/Stromal cells/Regulating the pivot through the shu points/Gastrointestinal hormones/Rats分类
医药卫生引用本文复制引用
陈峭,覃凤传,刘莹,梁梦月,谢胜,周晓玲,税典奎,覃婧,侯秋科,颜春艳,刘珊,刘礼剑.."以俞调枢"法对Cajal间质细胞缺失模型大鼠胃肠动力学主要指标的影响[J].中国全科医学,2018,21(9):1109-1113,5.基金项目
国家自然科学基金资助项目(81403383)——基于红外热成像技术及SCF/c-kit/ICC信号通路探讨"以俞调枢"法改善胃肠动力的作用机制 （81403383）
广西重点研发项目(桂科AB16380206)——"以俞调枢"法在胃肠动力障碍性疾病中的应用示范及其规范化研究 （桂科AB16380206）
