中国蔬菜Issue(12)：30-34,5.
野生番茄LA2157中热稳定抗根结线虫基因Mi-9候选基因的分离与过表达载体构建
Isolation of Heat-stable Candidate Gene Mi-9 for Resistance to Root-knot Nematode in Wild Tomato LA2157 and Construction of Over-expressive Vector
摘要
Abstract
Root-knot nematode disease is one of the important tomato soil-borne diseases.Breeding varieties with root-knot nematode resistance is an effective control method to deal with this disease.But at present,Mi-1 the only gene transferred to tomato culture with resistance to root-knot nematode loses its resistance when the soil temperature is over 28 ℃.In this study,wild tomato accession LA2157 with heat-stable resistance to root-knot nematode was used.According to the sequence information of Mi-1,we carried out homologous cloning on Mi-9 gene with heat-stable resistance to root-knot nematode and gained 2 candidate gene segments from LA2157.The candidate gene was ligated with the overexpression vector pBI121 by In-Fusion cloning technique.Electrophoresis and sequencing analysis proved that the recombinant vectors of the candidate gene were constructed.关键词
番茄/根结线虫/Mi-9基因/同源克隆/过表达载体构建Key words
Tomato/Root-knot nematode/Mi-9 gene/Homologous cloning/Over-experessive vector construction引用本文复制引用
王银磊,陈伟男,赵丽萍,周蓉,宋刘霞,余文贵,赵统敏..野生番茄LA2157中热稳定抗根结线虫基因Mi-9候选基因的分离与过表达载体构建[J].中国蔬菜,2017,(12):30-34,5.基金项目
国家自然科学青年基金项目(31401884),江苏省自然科学青年基金项目(BK20140739) （31401884）
